Biolegend, 667204, Purified anti-Cystatin C Antibody, 100μg

Cystatin C was initially identified in human urine and was named gamma-trace. It is a type II cystatin sub-family member. The cystatin family contains three subfamilies: Type 1 cystatins, also known as stefins, which are predominantly present in the cytosol and the nuclei; Type 2 cystatins are mainly secreted proteins; Type 3 cystatins are multi-domain proteins of high molecular weight (60-120 kD) and are also known as kininogens. Cystatin C inhibits cysteine proteases from the papain family and legumain. It forms tight non-covalent associations with its target enzymes. Cystatin C antagonizes TGF-β signaling in normal and cancer cells in vitro . In this process, Cystatin C binds to the TGF-β type II receptor and inhibits the binding of TGF-β. Cystatin C influences the progression of diseases such as, cancer metastasis, atherosclerosis, aortic aneurisms, emphysema, and arthritis. Also, it is associated with neurodegenerative disease and cerebral angiopathy. Human Cystatin C has the tendency to dimerize in order to produce very tight two-fold symmetric dimers. This amyloidogenic property suggests a mechanism for its aggregation in the brain arteries of elderly people with amyloid angiopathy. The L68Q mutant of human Cystatin C is responsible for conformational disease, which causes massive amyloidosis, cerebral hemorrhage, and death in young adults.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Endogenous protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2629541 (BioLegend Cat. No. 667204)
Structure: Cystatin C is a 13 kD protein which inhibits cystein proteases.
Distribution: Considered ubiquitously expressed, biological fluids, dendritic cells, and macrophages.
Biology Area: Cancer Biomarkers, Cell Biology
Antigen References: 1. Grubb A, et al. 1982. Proc. Nat. Acad. Sci. 79:3024.2. Janowski R, et al. 2001. Nat. Struct. Biol. 8:316.3. Sokol JP, Schiemann WP. 2004. Mol. Cancer Res. 2:183.4. Bird PI, et al. 2009. Nat Rev Immunol. 9:871.5. Kaur G, Levy E. 2012. Front Mol. Neurosci. 5:79.6. Xu Y, et al. 2014. J Biol Chem. 289:9730.7. Xu Y, et al. 2015. Immunol. Cell Biol. 5:442-51.
Gene ID: 1471
UniProt: View information about Cystatin C on UniProt.org
Clone: E4C6(2A10H7)
Regulatory Status: RUO
Other Names: Cystatin-C, Cystatin-3, Gamma-trace, Neuroendocrine basic polypeptide, Post-gamma-globulin, CST3
Isotype: Mouse IgG1