Biolegend, 667652, Purified anti-PRAS40/AKT1S1 Antibody, 100μg

Numerous growth factors and hormones have the capability to initiate the phosphoinositide 3-kinase signaling pathway, leading to the activation of downstream effector proteins such as the serine/threonine kinase AKT. A 40 kDa proline-rich protein called PRAS40 (also known as AKT1S1), which interacts with 14-3-3 proteins, is one known AKT substrate. In order to transduce AKT signals to the mTOR complex, PRAS40/AKT1S1 interacts with mTOR which regulates cell growth and cell cycle and acts as a negative regulator of autophagy. Inhibition of mTOR signaling promotes PRAS40/AKT1S1 binding to mTOR, which reduces mTOR activity. In PC12 cells, treatment with insulin-like growth factor-1 (IGF1) can cause phosphorylation of PRAS40/AKT1S1 by the PI3K/AKT signaling pathway. In insulin-deficient cells, PRAS40/AKT1S1 interacts with raptor in mTOR complex 1 (mTORC1) and prevents the cell cycle protein Rheb from activating the mTORC1 pathway. The inhibition of mTORC1 by PRAS40/AKT1S1 is relieved when AKT phosphorylates PRAS40/AKT1S1 at Thr246. Additionally, PRAS40/AKT1S1 is phosphorylated on Ser183, Ser212, and Ser221 by mTORC1, which releases it from mTORC1.
100μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant human PRAS40/AKT1S1 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For use of this antibody (clone W22030A) in immunocytochemistry (ICC), 4% PFA fixation followed by permeabilization with ice-cold methanol is recommended. This antibody (clone W22030A) is not suitable for use in immunohistochemistry (IHC).
Additional Product Notes: This antibody has been tested in knockout/knockdown models for WB and ICC.
RRID: AB_3662335 (BioLegend Cat. No. 667651) AB_3662335 (BioLegend Cat. No. 667652)
Structure: PRAS40/AKT1S1 is a 256 amino acid protein with a predicted molecular weight of 27 kD.
Distribution: Widely expressed with highest levels of expression in the liver and heart
Function: Negative regulator of mTORC1 and plays a role in nerve growth factor-mediated neuroprotection
Interaction: mTORC1, MTOR, MLST8, RPTOR
Cell Type: Neurons
Biology Area: Cell Biology, Cell Proliferation and Viability, Neuroscience, Signal Transduction
Molecular Family: Adaptor Proteins
Antigen References: Cantley LC. 2002. Science. 296: 1655-7. Kovacina KS, et al. 2003. J Biol Chem. 278:10189-94. Vander Haar E, et al. 2007. Nat Cell Biol. 9:316-23. Sancak Y, et al. 2007. Mol Cell. 25:903-15. Oshiro N, et al. 2007. J Biol Chem. 282:20329-39. Wang L, et al. 2008. J Biol Chem. 283:15619-27. Wang H, et al. 2012. Neurosci Lett. 516:105-9.
Gene ID: 84335
UniProt: View information about PRAS40 on UniProt.org
Clone: W22030A
Regulatory Status: RUO
Other Names: AKT1S1, AKT1 Substrate 1, PRAS40, 40 KDa Proline-Rich AKT Substrate, AKT1 Substrate 1, Proline-Rich AKT1 Substrate 1, MGC2865
Isotype: Rat IgG2a, κ