Biolegend, 671001, Purified anti-Annexin A2 Antibody, 25μg

Annexin A2 (ANXA2) is a member of the annexin family, which are calcium-dependent phospholipid-binding proteins that play a role in the regulation of cellular growth and signal transduction pathways. Annexin A2 forms a heterotetramer complex with p11(S100A10) protein on the plasma membrane which then co-localizes with CD44, tenascin-C, cathepsin B and tissue plasminogen activator (t-PA). This complex leads to the activation of growth factors, proteases, degradation of extracellular matrix (ECM) proteins, and results in an increase in cancer cell adhesion invasion, metastasis and tumor growth. Annexin A2 also increases cancer cell proliferation and cell survival via the MAPK/ERK1/2 pathway and involves in actin cytoskeletal rearrangement and regulates cancer cell migration.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Synthetic peptide corresponding to 10-15 amino acids surrounding Lys302 of Annexin A2
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per ml. For immunocytochemistry, a concentration range of 1 - 5 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone failed to immunoprecipitate Annexin A2 from whole cell extracts prepared from HeLa cells. Triton X-100 permeabilization failed to properly stain for ICC, methanol permeabilization is the preferred method.
RRID: AB_2814526 (BioLegend Cat. No. 671001) AB_2814527 (BioLegend Cat. No. 671002)
Structure: Annexin A2 is a 339 amino acid protein with a predicted molecular weight of 38 kD
Distribution: Ubiquitously expressed
Function: Promotes cancer cell survival, proliferation and migration
Antigen References: Zhang F, et al. 2009. J. Proteome. Res. 8(11):5041-7. Keklikoglou I, et al. 2015. Oncogene. 37:4867. Deng FY, et al. 2011. Mol. Cell. Proteomics. 11:M111
Gene ID: 302
UniProt: View information about Annexin A2 on UniProt.org
Clone: W18057A
Regulatory Status: RUO
Other Names: Annexin A2, Annexin II, Calpactin-1 Heavy Chain, Lipocortin II, P36, Placental Anticoagulant Protein IV, Protein I, ANX2L4, LPC2D, PAP-IV, Chromobinding 8, Epididymis Secretory Sperm Binding Protein, HEL-S-270
Isotype: Rat IgG2a, λ