Biolegend, 671502, Purified anti-SEC13 Antibody, 100μg

SEC13 functions as a component of the nuclear pore complex (NPC) involved in nucleo-cytoplasmic trafficking. At the ER, SEC13 is involved in the biogenesis of COPII-coated vesicles, which are required for protein export from the ER to either the Golgi complex or the ER-Golgi intermediate compartment (ERGIC). It also functions as a component of the GATOR2 complex, which acts as an amino acid-sensing branch of the TOR1 signaling pathway.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial-length human SEC13 recombinant protein at 123-322 a.a.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.05 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone failed IP testing when the target was pulled down and probed via WB.In addition, a nonspecific band was detected in the initial Western Blot screen; however, that band was titrated out at ~0.1 µg/mL. Nevertheless, the presence of the band indicates that the clone is not suitable for ICC testing.
RRID: AB_2814534 (BioLegend Cat. No. 671501) AB_2814535 (BioLegend Cat. No. 671502)
Structure: SEC13 is a 322 amino-acid protein with a predicted molecular weight of 39 kD.
Distribution: Localized to the nucleoplasm and vesicles; ubiquitously expressed.
Function: mTOR signaling; vesicle biogenesis
Biology Area: Protein Trafficking and Clearance
Antigen References: Swaroop A, et al. 1994. Hum. Mol. Genet. 3(8):1281-6. Wolfson RL, et al. 2017. Nature 543(7645): 438-442. Cho HJ, et al. 2014. EMBO J 33(20):2314-31. Chantranupong L, et al. 2014. Cell Rep. 9(1):1-8. Bar-Peled L, et al. 2013. Science. 340(6136):1100-6.
Gene ID: 6396
UniProt: View information about SEC13 on UniProt.org
Clone: W18034A
Regulatory Status: RUO
Other Names: SEC13-like protein 1, SEC13-related protein; nuclear pore and COPII coat complex component
Isotype: Rat IgG2a, κ