Biolegend, 672604, Purified anti-FBXL5 Antibody, 100μg

In metazoans, iron homeostasis is mediated at the systemic and cellular levels by the RNA-binding iron regulatory proteins IRP1 and IRP2.Under conditions where cellular iron is limiting, IRP1 and IRP2 stabilize the mRNA of genes involved in iron acquisition and mobilization and translationally repress genes involved in iron storage and export.  Under iron replete conditions, both IRPs are inactivated: IRP1 acquires an Fe-S cluster that inhibits RNA-binding activity, while IRP2 is targeted for proteasomal-mediated degradation by the E3 ubiquitin ligase FBXL5. Under iron-replete conditions Fe-S cluster biogenesis is disrupted, FBXL5 will also target IRP1 for degradation. FBXL5, or F-box and leucine-rich repeat protein 5 (FBXL5), is the E3 ubiquitin ligase component of the SCF FBXL5 complex. The complex contains SKP1, Cullin-1, RBX1, and FBXL5. When cytosolic iron is replete, FBXL5 recruits IRP2 to the SCF complex and promotes its ubiquitination and subsequent degradation. FBXL5 contains a hemerythrin-like domain that senses oxygen and iron availability. This sensing mechanism is linked to the stability of the FBXL5 protein itself: when cytosolic iron is available, iron binds to the hemerythrin domain and promotes the stability of the FBXL5 protein. When iron is deficient, the FBXL5 protein lacks the iron ligand, resulting in its degradation and activation of the IRP-mediated iron starvation response. Studies have also shown that FBXL5 can ubiquitinate and target the SNAIL1 transcription factor and disruption of this process promotes epithelialto mesenchymal transition.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human FBXL5
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml (1:500 - 1:2000). It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone may require prolonged exposure times to visualize proteins by western blot. We strongly recommend loading at least 30 µg of protein to facilitate detection.
Application References(PubMed link indicates BioLegend citation): Moroishi T, et al. 2014. J Biol Chem. 289: 16430. (WB) Chollangi S, et al. 2012. J Biol Chem. 287: 23710. (WB)
RRID: AB_2749876 (BioLegend Cat. No. 672603) AB_2749875 (BioLegend Cat. No. 672604)
Structure: FBXL5 is a 691 amino acid protein with a predicted molecular weight of 78.6 kD. The protein contains an N-terminal hemerythrin like domain, followed by a F-box domain and seven leurcine-rich repeats.
Distribution: Cytosol and nucleus; ubiquitous expression
Function: Systemic and cellular iron homeostasis
Interaction: IRP2, IRP1, SKP1, Cullin-1, RBX1, HERC2
Ligand/Receptor: Iron and oxygen
Biology Area: Cell Biology, Ubiquitin/Protein Degradation
Antigen References: Johnson NB, et al. 2017. J. Biol. Chem. 292: 15976 Vinas-Castells R, et al. 2014. Nucleic Acids Res. 42: 1079 Moroishi T, et al. 2014. J Biol Chem. 289: 16430. Ruiz JC, et al. 2013. J Biol Chem 288:552. Chollangi S, et al. 2012. J Biol Chem. 287: 23710. Moroishi T, et al. 2011. Cell Metab. 14:339 Salahudeen AA, et al. 2009. Science. 326: 722 Vashisht AA, et al. 2009. Science. 326: 718
Gene ID: 2634
UniProt: View information about FBXL5 on UniProt.org
Clone: 10F4H9D12
Regulatory Status: RUO
Other Names: F-BOX/LLR-repeat protein 5, FBL5
Isotype: Mouse IgG2a