Biolegend, 673052, Purified anti-ASCL1 (MASH1) Antibody, 100μg

ASCL1 (Achaete-Scute Family BHLH Transcription Factor 1), also known as MASH1, is a transcription factor that plays a crucial role in neurogenesis, particularly in the development of the nervous system. ASCL1 is a member of the basic helix-loop-helix (BHLH) transcription factor family and acts by binding to specific E-box sequences in the DNA to regulate the expression of target genes that are necessary for the cell fate determination, differentiation, and development. The protein is expressed in various tissues during development, including the developing spinal cord, cortex, and hindbrain. In the neural tube, ASCL1 influences the differentiation of neural progenitors into various types of neurons, including dopaminergic, serotonergic, and other interneurons. The loss of ASCL1 function during development can lead to neurological disorders such as Hirschsprung disease, congenital central hypoventilation syndrome, dysautonomia. ASCL1 has been found to be involved in the progression of certain cancers, such as lung and neuroendocrine tumors. In these tumors, ASCL1 is often overexpressed and contributes to tumorigenesis by promoting the survival and proliferation of cancer cells.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of human ASCL1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: WB - Quality tested ICC, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 10.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with either of the following: -Fixation buffer (Cat. No. 420801) with 0.5 % Triton X-100 -Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol For use in intracellular flow cytometry (ICFC), it is recommended to fix/permeabilize with Fixation/permeabilization with True-Phos™ Perm Buffer (Cat. No. 425401). Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) or True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) is not recommended.
RRID: AB_3699091 (BioLegend Cat. No. 673051) AB_3699091 (BioLegend Cat. No. 673052)
Structure: Contain a basic helix-loop-helix (BHLH) domain. This protein consists of 236 amino acids with a predicted molecular weight of 25.5 kD.
Distribution: Expressed in brain, liver, and pituitary gland. Subcellular localization is in the nucleus.
Function: Transcription factor that plays a key role in neuronal differentiation
Interaction: Form heterodimer with TCF3 and bind to DNA
Cell Type: Neurons
Biology Area: Neuroscience, Neuroscience Cell Markers, Synaptic Biology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: Pattyn A, et al. 2004. Nat Neurosci. 7:589-95. Castro DS, et al. 2011. Genes Dev. 25:930-45. Chanda S, et al. 2014. Stem Cell Reports. 3:282-96.
Gene ID: 429
UniProt: View information about ASCL1 on UniProt.org
Clone: W23121G
Regulatory Status: RUO
Other Names: Achaete-Scute Family BHLH Transcription Factor 1, Achaete-Scute Complex (Drosophila) Homolog-Like 1, HASH1, BHLHa46, ASH-1
Isotype: Rat IgG2a, κ