Biolegend, 676202, Purified anti-mouse CD204 Antibody, 100μg

CD204, also known as scavenger receptor A (SR-A) and the macrophage scavenger receptor (MSR), is one of the phagocytic pattern-recognition receptors (PRRs) expressed on macrophages and dendritic cells. CD204 was initially identified as a receptor mediating recognition and internalization of low-density lipoprotein (LDL) by macrophages and playing critical roles in atherogenesis. Recent studies have revealed that CD204 recognizes apoptotic cells, modified lipid proteins, and exogenous pathogen-associated molecular patterns (PAMPs), which results in the induction of innate immune and inflammatory responses. CD204 can act as a co-receptor for Toll-like receptors, such as TLR3, TLR4, or TLR9, to facilitate the expression of proinflammatory cytokines. CD204 has been implicated in several pathological processes such as Alzheimer’s disease, sepsis, ischemic injury, and coronary artery disease.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial mouse CD204 recombinant protein (79-458 a.a.) expressed in 293E cells.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: CD204 is polymorphic and clone 7G5C33 may not react with all mouse strains. In house testing with this clone showed no reactivity to C57BL/6 mouse spleen samples.The predicted molecular weight of CD204 is 50 kD, the observed molecular weight when clone 7G5C33 is used for western blot is ~70 kD. This is likely due to glycosylation of CD204 at multiple sites.
RRID: AB_2565623 (BioLegend Cat. No. 676202)
Structure: 485 amino acids with a predicted molecular weight of 50 kD and is a single-pass type II membrane protein.
Distribution: Plasma membrane, low-density lipoprotein particle, and endocytic vesicle emmbrane.
Function: CD204, a trimeric type II integral membrane glycoprotein, is predominantly expressed on macrophages and dendritic cells. CD204 plays important roles in lipid metabolism, host defense, and inflammatory responses.
Interaction: CD204 interacts with HSPA1A.
Biology Area: Cell Biology, Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: 1. Kelley JL, et al. 2014. Crit. Rev. Immunol. 34:241. 2. Saito Y, et al. 2014. J. Clin. Exp. Hematop. 54:59. 3. Jozefowski S, et al. 2014. Innate Immun. 20:826. 4. Haasken S, et al. 2013. J. Immunol. 191:1055. 5. Yu H, et al. 2012. Biochim. Biophys. Acta. 7:1192. 6. Yu X, et al. 2012. Oncoimmunology 5:770.
Gene ID: 20288
UniProt: View information about CD204 on UniProt.org
Clone: 7G5C33
Regulatory Status: RUO
Other Names: Macrophage scavenger receptor 1 (Msr1), Scavenger receptor type A (SR-A), SRA
Isotype: Rat IgG1, κ