Biolegend, 676751, Purified anti-FOXP3 Antibody, 25μg

Forkhead box protein P3 (FOXP3) is a transcription factor that plays a role in the development and function of regulatory T cells (Tregs), which are essential for maintaining immune tolerance and preventing autoimmune diseases. FOXP3 is responsible for the transcriptional regulation of genes involved in Treg differentiation and function, ensuring the immune system does not attack the body's own tissues. Mutations or deficiencies in the FOXP3 gene can lead to severe autoimmune disorders, such as immunodysregulation, polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), characterized by widespread inflammation and multi-organ damage. Additionally, FOXP3 has been implicated in cancer biology, where it can act as a tumor suppressor in certain contexts.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant Fragment of Human FOXP3
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested WB, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For use of this antibody in immunohistochemistry on formalin-fixed paraffin-embedded tissues (IHC-P), we recommend antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902), or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704). For use of this antibody in intracellular flow cytometry (ICFC), we recommend fixation and permeabilization using FOXP3 Fix/Perm Buffer Set (Cat. No. 421403), True-Phos™ Perm Buffer (Cat. No. 425401) or True-Nuclear™ Transcription Factor Buffer set (Cat. No. 424401).
RRID: AB_3699093 (BioLegend Cat. No. 676751) AB_3699093 (BioLegend Cat. No. 676752)
Structure: The FOXP3 protein is composed of 431 amino acids and has a molecular weight of approximately 47.2 kDa
Distribution: Nuclear
Function: • Regulatory T Cell Development: Essential for the development and function of regulatory T cells (Tregs), which maintain immune tolerance and prevent autoimmune diseases. • Transcriptional Regulation: • Acts as a transcription factor, binding to specific DNA regions to control the expression of genes involved in immune regulation. • Immune Response Modulation: Helps suppress the immune response to prevent excessive inflammation and autoimmunity
Interaction: • Transcription Factors: interacts with several transcription factors, including NFAT, AML-1, and c-Rel, to regulate gene expression. • Histone Deacetylases (HDACs): recruit class I histone deacetylases (HDAC1, HDAC2, and HDAC3) to target gene promoters, modulating chromatin structure and gene expression. • Co-repressors and Co-activators: It interacts with co-repressors like CTBP1 and co-activators such as TIP60 to fine-tune transcriptional regulation
Cell Type: Leukemia, Lymphocytes, T cells, Tregs
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Immuno-Oncology, Immunology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: Zhou Z, et al. 2008. Immunology Research. 42:19 Colamatteo A, et al. 2020. Front Immunol. 10:3136 Golzari-Sorkheh M, et al. 2023. Clin Exp Immunol. 213:13 Xie X, et al. 2015. PLoS Genet. 11:e1005251.
Regulation: • Transcriptional Control: Regulated by various transcription factors that bind to its promoter and enhancer regions. • Epigenetic Modifications: DNA methylation and histone modifications influence FOXP3 expression by altering chromatin structure. • Post-Translational Modifications: Phosphorylation and other modifications can affect FOXP3 stability and function. • TCR Signaling: T cell receptor (TCR) signaling pathways can modulate FOXP3 expression and activity
Gene ID: 50943
UniProt: View information about FOXP3 on UniProt.org
Clone: W23223B
Regulatory Status: RUO
Other Names: Scurfin, JM2, IPEX, PIDX
Isotype: Rat IgG2a, κ