Biolegend, 677052, Purified anti-HIF2A Antibody, 100μg

HIF2A is a transcription factor that mediates the cellular adaptive response to hypoxic conditions, orchestrating the transcription of a wide array of target genes to enable cellular survival and homeostatic maintenance in low-oxygen environments. At an organismal level, HIF2A is also involved in essential physiological processes such as erythropoiesis and angiogenesis. Under normoxic conditions, HIF2A undergoes prolyl hydroxylation which leads to its degradation by the proteasome. Oxygen starvation inhibits prolyl hydroxylation and prevents HIF2A degradation, leading to stabilization of the protein and translocation to the nucleus to alter gene expression. Dysregulation of HIF2A has been implicated in various pathological states including cancer, where it promotes tumor growth by enhancing the ability of tumor cells to survive hypoxic conditions within the tumor microenvironment.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of the C-terminus of human HIF2A
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody is not recommended for use in immunocytochemistry (ICC), intracellular flow cytometry (ICFC), or immunohistochemistry in formalin-fixed, paraffin-embedded tissues (IHC-P).
RRID: AB_3683496 (BioLegend Cat. No. 677051) AB_3683496 (BioLegend Cat. No. 677052)
Structure: HIF2A is an 870 amino acid protein with a predicted molecular weight of 96.5 kD.
Distribution: Cytoplasmic, translocates to nucleus upon stabilization
Function: Transcription factor
Interaction: Dimerizes with HIF1B upon hypoxic induction. Under normoxic conditions, undergoes prolyl hydroxylation by Von Hippel-Lindau (VHL)
Cell Type: Endothelial cells
Biology Area: Signal Transduction, Transcription Factors
Antigen References: Tian H, et al. 1997. Genes Dev. 11:72-82. Wiesener, et al. 1998. Blood. 92:2260-8. Giatromanolaki A and Harris AL 2001. Anticancer Res. 6B:4317-24. MacInnis MJ and Rupert JL. 2011. High Alt Med Biol. 12:133-9.
Gene ID: 2034
UniProt: View information about HIF2A on UniProt.org
Clone: W22144B
Regulatory Status: RUO
Other Names: Q99814, Hypoxia induficble factor 2-alpha (HIF2A, HIF2α), Endothelial PAS domain-containing protein 1 (EPAS1), class E basic helix-loop-helix protein 73 (bHLHe73), Member of PAS protein 2 (MOP2), PAS domain-containing protein 2 (PASD2), BHLHE73, HIF2
Isotype: Rat IgG2b, κ