Biolegend, 677301, Purified anti-Mac-2 (Galectin-3) Antibody, 50μg

Galectin-3 (galactose-specific lectin 3) is a unique member of the β-galactoside-binding lectin family molecule, which plays an important role in regulation of cell proliferation, differentiation, and tumor cell metastasis. Galectin-3 is mainly found in the cytoplasm, but it is also seen in the nucleus and can be secreted by the non-classical, secretory pathway. In general, secreted galectin-3 mediates cell migration, cell adhesion, and cell-cell interactions through its high affinity binding with galactose-containing glycoproteins on the cell surface. Cytoplasmic galectin-3 exhibits anti-apoptotic activity and regulates several signal transduction pathways, whereas nuclear galectin-3 has been associated with pre-mRNA splicing and gene expression. Recent studies also indicate the potential role of galectin-3 as a biomarker for acute and chronic heart failure.
50μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant full-length human Galectin-3.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For immunoprecipitation, the suggested use of this reagent is 1-5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Sharma M, et al. 2020. Circ Res. 127:335. PubMed
RRID: AB_2565901 (BioLegend Cat. No. 677301) AB_2565902 (BioLegend Cat. No. 677302)
Structure: 250 amino acids with a predicted molecular weight of approximately 26 kD.
Distribution: Localized in the nucleus, cytoplasm, cell surface, or extracellular matrix. Expressed by tumor cells, monocytes/macrophages, epithelial cells, Kupffer cells, and dendritic cells.
Function: Regulate cell proliferation, differentiation, adhesion, and anti-apoptosis.
Interaction: DMBT1, ITGA3, ITGB1, CSPG4, LGALS3BP, LYPD3, CYHR1, and UACA.
Ligand/Receptor: Variety of ligands such as IgE, IgA, galactose, casein kinase I, laminin, mucin, LAMPs, CD66, and etc.
Cell Type: Dendritic cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Cycle/DNA Replication, Cell Motility/Cytoskeleton/Structure, Immunology, Signal Transduction, Transcription Factors
Molecular Family: Adhesion Molecules
Antigen References: 1. Kim HR, et al. 1999. Cancer Res. 16:4148. 2. Reljic R, et al. 2004. Immunol. Lett. 93:51. 3. Takenaka Y, et al. 2004. Mol. and Cell. Biol. 24:4395. 4. Joo HG, et al. 2001. J Leuko. Biol. 4:555. 5. Gudowska M, et al. 2014. Pol. Merkur. Lekarski. 37:348. 6. Xin M, et al. 2015. Biomed. Pharmacother. 69:179. 7. Filipe MD, et al. 2015. Clin. Chim. Acta. 443:48. 8. Funasaka T, et al. 2014. Glycobiology 24:886.
Gene ID: 3958
UniProt: View information about Mac-2 on UniProt.org
Clone: M1606B04
Regulatory Status: RUO
Other Names: Mac-2, Gal-3, RL-29, galactose-specific lectin 3, CBP-35, L-34, εBP
Isotype: Rat IgG1, κ