Biolegend, 678003, Alexa Fluor® 594 anti-STAT3 Antibody, 100μg

STAT3 is an 88 kD member of the STAT (signal transducer and activators of transcription) protein family that is phosphorylated in response to a cytokine receptor-associated kinase activity. Phosphorylation of STAT3 induces nuclear translocation to activate transcription. STAT3 forms both homo- and heterotrimers and is involved in the activation of genes required for cell growth and apoptosis. STAT3 is also involved in gp130 signaling and binds to IL-6 response elements in various acute phase protein promoters. STAT3 is phosphorylated by signaling from IFNs, EGF, FGF, IL-5, HGF, LIF, and BMP2. STAT3 activity is inhibited by PIAS3 and GRIM-19 and can also be regulated by the Rac1 protein.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial human STAT3 recombinant protein (621-770 a.a.) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.1 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Application Notes: This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).
Product Citations: Cheng J, et al. 2022. iScience. 25:103588. PubMed
RRID: AB_2566584 (BioLegend Cat. No. 678003)
Structure: 770 amino acids with a predicted molecular weight of 88.1 kD that contains a C-terminal SH2 domain responsible for homo- or herterodimerization.
Distribution: Cytoplasm.
Function: Phosphorylated in response to cytokine signaling by receptor-associated kinases, translocates to the nucleus to act as a transcriptional activator, activates a wide variety of genes involved in cell growth and apoptosis, and is involved in gp130-related signaling.
Interaction: STAT3 forms a homodimer or a heterodimer with other STAT family members and interacts with IL31RA, NCOA1, PELP1, SIPAR, SOCS7, STATIP1, CAV2, DAPK3, EIF2AK2, PIAS3, and TMF1.
Cell Type: Embryonic Stem Cells, Mesenchymal Stem Cells, Neural Stem Cells
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells, Synaptic Biology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Lufei C, et al. 2003. EMBO J. 22:1325. 2. Deo D, et al. 2002. J. Biol. Chem. 277:21237. 3. Pfeffer L, et al. 1997. Science 276:1418. 4. Akira S, et al. 1994. Cell 77:63.
Gene ID: 6774
UniProt: View information about STAT3 on UniProt.org
Clone: 4G4B45
Regulatory Status: RUO
Other Names: Signal transducer and activator of transcription 3, Acute-phase response factor (APRF), HIES, ADMIO
Isotype: Mouse IgG1, κ