Biolegend, 678902, Purified anti-C2H2 zinc finger protein phospho linker region (HpTGEKP) Antibody, 100μg

C2H2 zinc finger proteins (ZFPs) are DNA-binding transcription factors that regulate a variety of biological processes such as cellular growth, differentiation, and proliferation. A zinc finger protein can contain several tandems of clustered zinc finger motifs responsible for DNA binding. However, stable ZFP binding to DNA requires the conserved linker sequences that join adjacent zinc fingers. These linkers participate in wrapping of the ZFP around the double helix of DNA, which is required for efficient binding. Most of the ZFP linkers contain the same amino acid sequence HTGEKP, which has been shown to be phosphorylated at the threonine residue by TOPK/PBK during mitosis. Phosphorylation on the linker sequence of ZFP leads to the inactivation of DNA binding activity.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Peptide (HQRIH(pT)GEKPYKC) conjugated to KLH.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For immunocytochemistry, a concentration range of 1.0 - 5.0 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2566052 (BioLegend Cat. No. 678902)
Structure: Conserved linker region (HTGEKP) of C2H2 zinc finger proteins.
Distribution: Mitotic cells.
Function: The linker region (HTGEKP) of C2H2 zinc finger proteins facilitate stable DNA binding and is highly conserved among these zinc finger proteins. During mitosis, C2H2 zinc finger proteins are phosphorylated on the threonine residue of the linker peptide, which result in the inactivation of their DNA binding activity.
Biology Area: Cell Biology, Cell Cycle/DNA Replication, Transcription Factors
Molecular Family: Phospho-Proteins
Antigen References: 1. Dovat S, et al. 2002. Genes Dev. 16:2985. 2. Rizkallah R, et al. 2011. Cell cycle 10:3327. 3. Rizkallah R, et al. 2015. Oncotarget 6:1446.
Gene ID: NA
UniProt: View information about C2H2 zinc finger proteins on UniProt.org
Clone: 7F8C76
Regulatory Status: RUO
Isotype: Mouse IgG1, κ