Biolegend, 679102, Purified anti-CARD9 Antibody, 100μg

Caspase recruitment domain-containing protein 9 (CARD9) is a member of the CARD protein family, which is known to play regulatory roles in cell apoptosis. CARD9 is an adaptor protein that mediates the signaling downstream of C-type lectin receptors (CLRs), such as Dectin-1, Dectin-2, and Mincle. CARD9 also participates in the signaling downstream of the Retinoic acid-inducible gene I receptor (RIG-1) family. After stimulation of the receptors, CARD9 is phosphorylated and forms a trimolecular complex with B cell leukemia-lymphoma 10 (Bcl10) and mucosa-associated lymphoid tissue 1 (Malt1), which activates the canonical NF-κB pathway and leads to the induction of proimflammatory cytokines, including IL-6, IL-1β, and tumor necrosis factors-alpha (TNFs). CARD9 has been suggested to play an important role in antifungal host defense, as the CARD9 deficient mice are more susceptible to pathogenic fungi infections.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human CARD9 peptide (LRKMQKGWRQGEEDRENT) conjugated to KLH.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. For immunocytochemistry, a concentration range of 1.0 - 5.0 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Zhu Q, et al. 2023. Front Cardiovasc Med. 10:1125717. PubMed
RRID: AB_2566076 (BioLegend Cat. No. 679102)
Structure: 536 amino acids with a predicted molecular weight of 62.2 kD. Contains a N-terminal CARD domain responsible for interacting with other CARD-containing proteins.
Distribution: Cytoplasm.
Function: CARD9 is a scaffold molecule that mediates the assembly of a signaling complex containing BCL10 and MALT1. CARD9 plays an important role in processes such as apoptosis, NF-κB activation, and host defense against pathogens.
Interaction: Interacts with BCL10, NOD2, RIPK2, and VHL.
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Innate Immunity, Neuroscience, Signal Transduction
Antigen References: 1. Jia XM, et al. 2014. J. Exp. Med. 211:2307. 2. Roth S, et al. 2014. Nat. Immunol. 15:538. 3. Yamamoto H, et al. 2014. Infect Immun. 4:1606. 4. Shenderov K, et al. 2013. J. Immunol. 190:5722. 5. Drummond RA, et al. 2011. Eur. J. Immunol. 41:276. 6. Goodridge HS, et al. 2009. J. Immunol. 182:1146.
Gene ID: 64170
UniProt: View information about CARD9 on UniProt.org
Clone: 13D9C60
Regulatory Status: RUO
Other Names: Caspase Recruitment Domain-Containing Protein 9, CANDF2, hCARD9
Isotype: Mouse IgG2a, κ