Biolegend, 679702, Purified anti-IGFBP-3 Antibody, 100μg

The insulin-like growth factor system includes two receptors (IGF-1R and IGF-2R), two ligands (IGF-1 and IGF-2), and seven binding proteins (IGFBP1-7). The binding proteins are produced in the liver and bind to the IGFs with different affinities, thus regulating the interaction of the IGFs with their receptors. Insulin-like growth factor binding protein-3 (IGFBP-3) is the major serum carrier protein for IGF proteins. IGFBP-3 sequesters IGF in a stable ternary complex with the acid-labile subunit (ALS). IGFBP-3 binds IGF-1 with high affinity and specificity and modulates IGF-1 action. IGFBP-3 undergoes limited enzymatic proteolysis to generate small N-terminal fragments. These proteolytic products lose their affinity for IGF-1 and IGF-2. Nevertheless, they preserve IGF independent activity. In that sense, a 16 kD fragment (1-95-IGFBP-3) and the full length molecule can potentiate apoptosis in colorectal epithelial cells, and this apoptotic process is associated with the inhibition of TNF-α-induced NF-κB activity. In addition, IGFBP-3 has been detected in the cell nucleus and interacts with 9-cis retinoic acid receptor-alpha (RXR-α), which is a nuclear receptor that is required for the apoptosis-inducing effects of IGFBP-3. IGFBP-3R physically interacts and activates caspase-8 and mediates IGFBP-3-induced apoptosis and tumor suppression.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human IGFBP-3, amino acids (Gly28-Glu291) (Accession# X64875) with an N-terminal Met, was expressed in 293E cells.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2566243 (BioLegend Cat. No. 679702)
Structure: 291 amino acids with a predicted molecular weight of approximately 32 kD. The glycosylated form migrates at approximately 45-50 kD by SDS-PAGE.
Distribution: IGFBP-3 is expressed by most of the tissues and is present in plasma.
Function: Transports insulin like growth factors (IGFs) and protects them from rapid clearance and/or degradation as well as modulating IGFs bioavailability. IGFBP-3 induces apoptosis in vitro and suppresses tumors in vivo. IGFBP-3 is induced by growth hormones, TGF-β, vitamin D, retinoids, and anti-estrogens.
Interaction: Interacts with XLKD1, IGF2, IGF1, ALS, HN, and TMEM219.
Ligand/Receptor: IGFBP-3R (TMEM219).
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Signal Transduction
Antigen References: 1. Cohen P, et al. 1992. J. Clin. Endocrinol. Metab. 75:1046. 2. Lee KW and Cohen P. 2002. J. Endocrinol. 175:33. 3. Yan X, et al. 2004. J. Biol. Chem. 279:53232. 4. Shahjee H, et al. 2008. Growth Horm. IGF Res. 18:188-97. 5. Berrigan D, et al. 2009. Growth Horm. IGF Res. 2:146. 6. Ingermann AR, et al. 2010. J. Biol. Chem. 285:30233. 7. Forbes BE, et al. 2012. Front. Endocrinol. 3:38. 8. Baxter RC, et al. 2014. Nat. Rev. Cancer 14:329.
Gene ID: 3486
UniProt: View information about IGFBP-3 on UniProt.org
Clone: M6501G03
Regulatory Status: RUO
Other Names: Insulin-like growth factor binding protein 3, IGF-Binding Protein 3, Growth Hormone-Dependent Binding Protein, Binding Protein 29, IBP3, BP-53, IGFBP3
Isotype: Mouse IgG1, κ