Biolegend, 680603, Direct-Blot™ HRP anti-V5-tag Antibody, 100microl

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. The V5 tag is a 14 amino acid peptide derived from a small epitope (Pk) on the P and V proteins of simian virus 5 (SV5), a member of the paramyxovirus family. Because of its small size, the addition of a V5 tag sequence (GKPIPNPLLGLDST) to a given gene creates a stable fusion product that does not appear to interfere with the biochemical properties of the protein. Many recombinant proteins have been engineered to express a short sequence derived from the V5 molecule known as V5-tag.
100microl
Verified Reactivity: Epitope tag
Reported Reactivity: Species independent
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation: The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:2000. The optimal dilution should be determined by titration for each individual assay of interest. 25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.
RRID: AB_2734532 (BioLegend Cat. No. 680604) AB_2572008 (BioLegend Cat. No. 680603)
Structure: 14 amino acid peptide (GKPIPNPLLGLDST)
Function: The V5 tag can be used to detect the expression of recombinant proteins in bacteria, yeast, insects, and mammalian cell systems.
Biology Area: Cell Biology
Antigen References: 1. Uhlen M, et al. 1990. Meth Enzymol. 185:129. 2. Hanke T, et al. 1995. J. Virol. Methods 53:149. 3. Southern JA, et al. 1991. J. Gen. Virol. 72:1551. 4. Thomas SM, et al. 1988. Cell 54:891.
Gene ID: NA
UniProt: View information about V5-tag on UniProt.org
Clone: 7/4
Regulatory Status: RUO
Other Names: GKPIPNPLLGLDST epitope tag
Isotype: Mouse IgG2b, κ