Biolegend, 680802, Purified anti-TRAF3 Antibody, 100μg

TNFR-associated factor 3, also known as TRAF3, is a member of the TNF receptor associated factor (TRAF) protein family and was originally identified as an adaptor molecule binding to the cytoplasmic tail of CD40. The engagement of CD40 with its ligand recruits the complex, which consists of TRAF3, TRAF2, and c-IAP1/2. This results in the stabilization of NF-κB inducing kinase (NIK). NIK phosphorylates the IKKα complex and subsequently leads to the activation of the RelB/p52 complex. TRAF3 is involved in the Toll-like receptor (TLR) mediated activation of proinflammatory genes such as type I interferons and IL-10. Pathogens detected by TLR3 and TLR4 recruit the adaptor protein TRIF. In turn, this activates IRF3 via the TRAF3-TBK1-IKKe complex. TRAF3 has been shown to play important roles in the regulation of innate immune response, antibody class switching, and B cell survival.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial TRAF3 recombinant protein (1-200 a.a.) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedKO/KD-WB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2566432 (BioLegend Cat. No. 680802)
Structure: 568 amino acids with a predicted molecular weight of 64.5 kD. Contains a C-terminal MATH/TRAF domain responsible for the binding to receptor cytoplasmic domains.
Distribution: Cytoplasm.
Function: TRAF3 mediates the signal transduction from TNF receptors and Toll-like receptors, leading to the activation of NF-κB, MAP kinases, or IRF3. TRAF3 is involved in the regulation of anti-viral response and B-cell survival.
Interaction: TRAF3 forms a homodimer. TRAF3 also forms heterodimers with TRAF2 and TRAF5. TRAF3 also interacts with CD40, TICAM1, TRAFD1, BIRC2, BIRC3, TLR4. TRIP, and TBK1.
Biology Area: Cell Biology, Immunology, Innate Immunity, Signal Transduction
Antigen References: 1. Hildebrand JM, et al. 2011. Immunol. Rev. 244:55. 2. Hu H, et al. 2013. Nature 494:371. 3. Jenkinson SR, et al. 2013. Proc. Natl. Acad. Sci. USA 110:21107. 4. Lin WW, et al. 2013. Front Immunol. 4:477. 5. Yi Z, et al. 2014. Cytokine Growth Factor Rev. 25:147. 6. Yi Z, et al. 2014. Nat. Immunol. 15:866.
Gene ID: 7187
UniProt: View information about TRAF3 on UniProt.org
Clone: 9D7C15
Regulatory Status: RUO
Other Names: TNF receptor-associated factor 3, CAP1, CD40 receptor-associated factor 1 (CRAF1), CD40-binding protein (CD40BP), LMP1-associated protein 1 (LAP1), IIAE5
Isotype: Mouse IgG1, κ