Biolegend, 681102, Purified anti-FGF-19 Antibody, 100μg

FGF-19 belongs to a subfamily of the fibroblast growth factors (FGFs) called endocrine FGFs, which consists of FGF-19, FGF-21, and FGF-23. Unlike other subfamily of FGFs, FGF-19 has a low binding affinity to heparin sulfate and functions as a circulating hormone with effects on distant tissues. FGF-19 has reduced binding affinity to the FGF receptors and the interaction requires two necessary cofactors that are α-Klotho and β-Klotho. In the ileum, FGF-19 expression is initiated by bile acids-induced activation of farnesoid X receptor (FXR). In response to bile acid stimulation, FGF-19 is secreted and enters the portal venous circulation, which subsequently down-regulates hepatic bile acid synthesis through repression of the CYP7A1 gene encoding cholesterol 7-alpha-monooxygenase. The impaired regulation of bile acids by FGF-19 leads to bile acid diarrhea also known as idiopathic bile acid malabsorption. FGF-19 has also been demonstrated to regulate carbohydrate and lipid metabolism.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial human FGF-19 recombinant protein (25-216 a.a.) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2566443 (BioLegend Cat. No. 681102)
Structure: Growth factor. 216 amino acids with a predicted molecular weight of 24 kD.
Distribution: Secreted.
Function: FGF-19 functions as a hormone, regulating homeostasis of bile acid, glucose, lipids, phosphate, and vitamin D.
Interaction: Interacts with heparian, α-Klotho, and β-Klotho.
Ligand/Receptor: FGFR1, FGFR2, FGFR3, and FGFR4.
Biology Area: Cell Biology
Molecular Family: Growth Factors, Cytokines/Chemokines
Antigen References: 1. Xie MH, et al. 1999. Cytokine 11:729.2. Harmer NJ, et al. 2004. Biochemistry 43:629.3. Goetz R, et al. 2007. Mol. Cell Biol. 27:3417.4. Wu X, et al. 2008. J. Biol. Chem. 283:33304.5. Song KH, et al. 2009. Hepatology 49:297.6. Turner N, et al. 2010. Nat. Rev. Cancer 10:116.
Gene ID: 9965
UniProt: View information about FGF-19 on UniProt.org
Clone: 13D9C80
Regulatory Status: RUO
Other Names: Fibroblast growth factor 19
Isotype: Mouse IgG1, κ