Biolegend, 681702, Purified anti-Siglec-11 Antibody, 100μg

Siglec-11 is a 686 amino acid single-pass type I membrane protein that contains one Ig-like V-type domain and three Ig-like C2-type domains. In humans, Siglec-11 is widely expressed on tissue macrophages, which include microglia in the brain. No expression of Siglec-11 is seen in the brain compartment of non-human primates. Siglec-11 functions as an adhesion molecule that preferentially binds to α-2, 8-linked sialic acid trimers and mediates sialic acid binding to cells. It is a negative regulator of microglia, both related to inflammation and phagocytic function. In murine microglial cells with ectopic expression of human Siglec-11, stimulation of Siglec-11 could induce the transcription of the pro-inflammatory mediator genes, such as IL-1β and nitric oxide synthase-2. Phagocytosis of apoptotic neuronal material has shown to be reduced in Siglec-11 expressing microglia. Co-culture of Siglec-11 expressing microglia and neurons demonstrated neuroprotective functions of Siglec-11.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant Siglec-11 fused to Fc region of human IgG.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Angata T, et al. 2002. J. Biol. Chem. 277:24466.
RRID: AB_2566496 (BioLegend Cat. No. 681702)
Structure: 698 amino acids, predicted molecular weight of 66 kD. Apparent molecular weight is 100-130 kD due to N-glycosylation.
Distribution: Plasma membrane.
Function: Adhesion molecule that mediates sialic-acid dependent binding to cells. The cytosolic domain of Siglec-11 is phosphorylated at tyrosine residue(s) upon pervanadate treatment of cells and then it recruits the protein-tyroisine phosphatases SHP-1 and SHP-2. Such interaction induces inhibitory cellular signals in immune response.
Interaction: Interacts with PTPN6/SHP-1 and PTPN11/SHIP-2 upon phosphorylation.
Ligand/Receptor: α-2,8-linked sialic acid trimers.
Biology Area: Cell Adhesion, Cell Biology, Immunology
Molecular Family: Adhesion Molecules, Siglec Molecules
Antigen References: 1. Angata T, et al. 2002. J. Biol. Chem. 277:24466. 2. Hayakawa T, et al. 2005. Science 309:1693. 3. Kopatz J, et al. 2014. Glycobiology 9:794. 4. Wang Y, et al. 2010. J. Neurosci. 30:3482. 5. Linnartz B, et al. 2010. Int. J. Alzheimers Dis. 2010.
Gene ID: 114132
UniProt: View information about Siglec-11 on UniProt.org
Clone: 4C4
Regulatory Status: RUO
Other Names: Sialic acid-binding Ig-like lectin 11, Sialic acid-binding lectin 11
Isotype: Mouse IgG2b, κ