Biolegend, 682952, Purified anti-HAGH Antibody, 100μg

Hydroxyacylglutathione hydrolase, mitochondrial or GLO2, is integral to cellular detoxification and metabolic regulation. It operates within the glyoxalase system, a critical pathway for neutralizing methylglyoxal, a highly reactive and cytotoxic byproduct of glycolysis. The glyoxalase system includes two primary enzymes: Lactoylglutathione lyase (GLO1) and HAGH/GLO2. GLO1 catalyzes the reaction between methylglyoxal and glutathione (GSH) to form S-D-lactoylglutathione. Subsequently, HAGH/GLO2 hydrolyzes S-D-lactoylglutathione to form D-lactate and regenerates GSH. This detoxification is crucial as methylglyoxal can cause significant cellular damage by glycation, which impairs the function of proteins, nucleic acids, and lipids. By mitigating this damage, HAGH helps protect cellular components and maintain cellular integrity.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of human HAGH (GLO2)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody (clone W22099D) is not suitable for immunocytochemistry (ICC). For immunohistochemistry on formalin-fixed parafilm-embedded tissue (IHC-P), the recommended antigen retrieval is either Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704) or Citrate Buffer (Cat. No.420902).
RRID: AB_3662337 (BioLegend Cat. No. 682951) AB_3662337 (BioLegend Cat. No. 682952)
Structure: HAGH/GLO2 is a 308 amino acid protein with a predicted molecular weight of 34 kD.
Distribution: Highly expressed in liver and kidney. Localizes in the cytosol and/or mitochondrial matrix.
Function: Catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid
Biology Area: Cell Biology, Mitochondrial Function
Molecular Family: Enzymes and Regulators
Antigen References: Rabbani N, et al. 2014. Biochem Soc Trans. 42:419-24. Bangel FN, et al. 2015. Prog Neuropsychopharmacol Biol Psychiatry. 59:105-110. Gaffney DO, et al. 2020. Cell Chem Biol. 27:206-213.
Gene ID: 3029
UniProt: View information about HAGH on UniProt.org
Clone: W22099D
Regulatory Status: RUO
Other Names: HAGH1, Hydroxyacylglutathione Hydrolase, Hydroxyacylglutathione Hydrolase, Mitochondrial, Glyoxalase II, GLO2, GLX2, GLO-2, Q16775
Isotype: Rat IgG2a, κ