Biolegend, 683602, Purified anti-CCL24 (Eotaxin-2) Antibody, 100μg

CLL24 was initially cloned from a library of activated macrophages. Using human eosinophils, it was shown that it binds to the receptor of eotaxin-1 (CCL11) and MCP-4 (CCL13). In addition, CCL24 shares the CCR3 receptor with CCL26, CCL5, and CCL7. Eotaxin/CCR3 expression has been studied extensively in the pathogenesis of asthma and allergies. The eotaxin/CCR3 axis has been associated to inflammatory and autoimmune disorders including inflammatory bowel disease, multiple sclerosis, eosinophilic esophagitis, and rheumatoid arthritis. CCL24 has also been associated to neovascular age-related macular degeneration.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant Human CCL24
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2572025 (BioLegend Cat. No. 683602)
Structure: 93 amino acids with a predicted molecular weight of 10.4 KD.
Distribution: CCL24 is expressed by epithelial cells, airway smooth muscle cells, and vascular endothelial cells. In addition, eotaxins are produced by cells isolated by bronchial alveolar lavage, including macrophages, monocytes, basophils, eosinophils, and lymphocytes.
Function: CCL24 attracts CCR3+ cells such as eosinophils, mast cells, basophils, and Th2 cells that play a role in allergic disorders. IL-4 and IL-13 induce CCL11 and CCL24 in esophagus and colonic epithelial cell, and IL-13 induces the production of CCL24 in dendritic cells and alveolar macrophages. In addition, CCL24 is induced by IL-33 in lung and by IL-4 and LPS in monocytes and macrophages. The expression of CCL24 by alveolar epithelial cells requires histamine and its receptor H2R.
Interaction: Eosinophils, mast cells, Th2 lymphocytes, basophils, human dendritic cells, synovial tissue macrophages, lung epithelial cells, and airway smooth muscle cells.
Ligand/Receptor: CCR3
Cell Type: Epithelial cells, Endothelial cells, Macrophages, Monocytes, Basophils, Eosinophils, Lymphocytes
Biology Area: Cell Biology, Immunology, Innate Immunity
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Patel VP, et al. 1997. J. Exp. Med. 185:1163.2. Beaulieu S, et al. 2002. J. Immunol. 169:2925.3. Watanabe K, et al. 2002. J. Immunol. 168:1911.4. Ablin JN, et al. 2010. Clin. Exp. Immunol. 161:276.5. Manousou P, et al. 2010. Clin. Exp. Immunol. 162:337.6. Stolarski B, et al. 2010. J. Immunol. 185:3472.7. Crapster-Pregont M, et al. 2012. J. Allergy Clin. Immunol. 129:1621.8. Swartzendruber JA, et al. 2012. J. Immunol. 188:536.
Gene ID: 6369
UniProt: View information about CCL24 on UniProt.org
Clone: A15037C
Regulatory Status: RUO
Other Names: Eotaxin2, MPIF2, MPIF-2
Isotype: Mouse IgG2b, κ