Biolegend, 683854, Alexa Fluor® 647 anti-Tau Phospho (Thr231) Antibody, 100μg

Paired helical filaments (PHFs) are the major building blocks of neurofibrillary lesions in Alzheimer's disease brains and are composed of hyperphosphorylated Tau protein. Predominantly expressed in axons, alternatively spliced forms of Tau comprise a family of microtubule-associated proteins that normally promote and stabilize the assembly of microtubules. PHF-Tau differs from normal Tau by its abnormal hyperphosphorylation, which results in decreased binding of Tau to microtubules. The decreased affinity of PHF-Tau for microtubules, coupled with reduced levels of normal Tau, destabilizes microtubules leading to an impairment of axonal transport, neuronal death and the aggregation of PHFs. Therefore, hyperphosphorylation of Tau is believed to be a key event in the pathogenesis of Alzheimer's disease.
100μg
Verified Reactivity: Human, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic human Tau peptide phosphorylated at Thr231
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: IHC-P - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: For use of this antibody in immunohistochemistry on FFPE tissues (IHC-P), we recommend Citrate buffer (Cat. No. 420902) for antigen retrieval.
RRID: AB_3675195 (BioLegend Cat. No. 683853) AB_3675195 (BioLegend Cat. No. 683854)
Structure: Tau proteins constitute a family of six isoforms with the range from 352-441 amino acids with molecular weights from 55 kD to 78 kD.
Distribution: Microtubules associated in axons of neurons of the central nervous system
Function: Maintain stability of microtubules in neurons. It is regulated by phosphorylation.
Interaction: Tubulin microtubules
Cell Type: Neurons
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Cell Structure, Neurodegeneration, Neuroscience Cell Markers, Protein Misfolding and Aggregation
Molecular Family: Intermediate Filaments, Tau
Antigen References: Lasagna-Reeves CA, et al. 2012. FASEB J. 26:1946-1959. Mitchell TW, et al. 2000. J Histochem Cytochem. 48:1627-1637. Hong M, et al. 1997. J Biol Chem. 272:25326-25332. Bramblett GT, et al. 1993. Neuron. 10:1089-1099.
Gene ID: 4137
UniProt: View information about Tau Phospho Thr231 on UniProt.org
Clone: A23009A
Regulatory Status: RUO
Other Names: Microtubule-associated protein tau, PHF-tau, paired helical filament-tau, neurofibrillary tangle protein, microtubule-associated protein tau, isoform 4, G protein beta1/gamma2 subunit-interacting factor 1, MAPT
Isotype: Mouse IgG2b, κ