Biolegend, 686902, Purified anti-ERK1/2 Antibody, 100μg

ERK1/2 are members of the mitogen-activated kinases (MAPKs) that are serine/threonine protein kinases. ERK1/2 can be activated by a range of extracellular stimuli, such as mitogen, growth factors, neurotransmitters, chemokines, and cytokines, through receptor tyrosine kinases (RTK), G protein-coupled receptors (GPCRs), or protein kinase C (PKC). Upon stimulation, ERK1/2 are phosphorylated by the upstream kinase MEK on residues Thr202 and Tyr204 and in turn phosphorylate many other downstream molecules that are involved in a range of cellular processes such as cell proliferation, differentiation, motility and cell death.
100μg
Verified Reactivity: Human, Mouse, Drosophila
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial human ERK1 recombinant protein (180-379 a.a.) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested KO/KD-WB, ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 – 2.0 µg/ml in human, mouse, and Drosophila. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Reactivity to Drosophila was only verified with the purified format. The drosophila homolog of mammalian ERK1/2 is the 43 kD mitogen-activated protein kinase ERK-A.
Product Citations: Chyuan IT, et al. 2024. J Biomed Sci. 33:31. PubMed Zhang A, et al. 2022. EMBO J. 41:e109187. PubMed McFall T, et al. 2021. Cell Rep. 37:110096. PubMed McFall T, et al. 2022. NPJ Precis Oncol. 6:86. PubMed Zhang Q, et al. 2021. Transl Res. 235:129. PubMed Bent EH, et al. 2021. Nat Commun. 12:6218. PubMed Lucas ED, et al. 2020. Cell Reports. 33(2):108258. PubMed
RRID: AB_2861071 (BioLegend Cat. No. 686901) AB_2629535 (BioLegend Cat. No. 686902)
Structure: ERK1 and ERK2 encode 42 and 44 kD protein kinases and share 85% homology.
Distribution: Nucleus and cytoplasm.
Function: ERK1/2 are broadly expressed protein kinases that are involved in proliferation, differentiation, motility, and cell death. In response to extracellular stimuli, ERK1/2 are phosphorylated at Thr202 and Tyr204 and in turn transduce signals to downstream substrates.
Interaction: Interacts with ADAM15, ARRB2, CANX, DAPK1, HSF4, IER3, MAP2K1/MEK1, MORG1, NISCH, SGK1, TPR, CDKN2AIP, PEA15, and MKNK2.
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Signal Transduction
Molecular Family: Protein Kinases/Phosphatase
Antigen References: 1. Futran AS, et al. 2013. Curr. Biol. 23:R972. 2. Mendoza MC, et al. 2011. Trends Biochem. Sci. 36:320. 3. Chambard JC, et al. 2007. Biochim. Biophys. Acta. 1773:1299. 4. Roux PP, et al. 2004. Microbiol. Mol. Biol. Rev. 68:320. 5. Torii S, et al. 2004. J. Biochem. 136:557. 6. Clark EA, et al. 1996. J. Biol. Chem. 271:14814.
Gene ID: 55955594
UniProt: View information about ERK1/2 on UniProt.org
Clone: W15133B
Regulatory Status: RUO
Other Names: Extracellular signal-regulated kinase 1/2, Mitogen-activated protein kinase MAPK3/MAPK1, p44ERK1/p42ERK2, p44-MAPK/p42MAPK, PRKM3/PRKM2, ERK
Isotype: Rat IgG1, κ