Biolegend, 687102, Purified anti-human Ikaros Antibody, 100μg

Ikaros, also known as IKZF1, belongs to a family of zinc-finger DNA-binding proteins that regulate lymphocyte differentiation. It has the ability to form homo- and heterodimers with other members of the Ikaros family, which include Aiolos and Helios. Ikaros can be found on a majority of fetal and adult hematopoietic cell types such as T- and B-cells, natural killer cells (NKs), monocytes/macrophages, and dendritic cells. Ikaros is a tumor suppressor and its absence or reduced expression has been reported in several leukemias.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human Ikaros peptide (431-477 aa) conjugated to KLH Freund's adjuvant.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ChIP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per mL. The recommended starting antibody to chromatin ratio is 4 µg of antibody and 5 µg of chromatin per IP. Optimal antibody to chromatin ratio should be determined by the end user. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: There are eight splicing variants with predicted MW 58, 53, 48(2), 43, 41, 31, 24 KDa. This antibody might recognize some of the isoforms.
RRID: AB_2616940 (BioLegend Cat. No. 687102)
Structure: Zinc-finger DNA binding protein, homo/heterodimer, 57.5 kD
Distribution: Fetal and adult hematopoietic cells.
Function: Regulates lymphocyte differentiation.
Interaction: DNA.
Cell Type: B cells, Dendritic cells
Biology Area: Cell Biology, Chromatin Remodeling/Epigenetics, Immunology, Signal Transduction, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Wargnier A, et al. 1995. Proc. Natl. Acad. Sci. USA 92:6930. 2. Tonnelle C, et al. 2009. Mol. Immunol. 46:1736. 3. Wang H, et al. 2014. Pediatr. Blood Cancer 61:2230.
Gene ID: 10320
UniProt: View information about Ikaros on UniProt.org
Clone: 16B5C71
Regulatory Status: RUO
Other Names: IK1, IKZF1
Isotype: Mouse IgG2a, κ