Biolegend, 687802, Purified anti-14-3-3 ζ/δ Antibody, 100μg

In mammals, proteins of the 14-3-3 family include isoforms β, γ, ε, σ, ζ, τ, and η. 14-3-3 δ isoform is the phosphorylated form of 14-3-3 ζ. These highly conserved proteins form homo- or heterodimers with many transcription factors, metabolic enzymes, kinases, phosphatases, and other signaling molecules, generally through a phospho-Ser/Thr motif. The binding regulates the location, stability, interaction with other molecules, and phosphorylation status of the target proteins which suggests the important roles for these 14-3-3 family proteins on regulation of cellular functions and responses. Growing data demonstrate that the specific temporal and spatial distributions of 14-3-3 family proteins are critical for developmental processes; and in some cancers, the regulation of these proteins are different from healthy controls. 14-3-3 ζ interacts with IRS1 protein and is involved in the PI3K-Akt signaling pathway.
100μg
Verified Reactivity: Human, Mouse, Rat, Drosophila
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Full length human 14-3-3 ζ/δ recombinant protein expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 – 2.0 µg/ml in human, mouse, and rat and 0.25 – 2.0 µg/ml in Drosophila. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: 14-3-3 family members share a high homology in amino acid sequences. Hence, this clone may recognize other 14-3-3 family members with predicted MW of 27, 28kD.Reactivity to Drosophila was only verified with the purified format.
RRID: AB_2617006 (BioLegend Cat. No. 687802)
Structure: 245 amino acids with a predicted molecular weight of 27.7 kD.
Distribution: Cytoplasm.
Function: 14-3-3 ζ/δ is an adaptor protein, binding to a large number of Ser/Thr phosphorylated proteins. It participates in several signaling pathways and regulates the activity of the binding partners.
Interaction: Forms a homooligomer or a heterodimer with other isoforms. Interacts with CDK16, BSPRY, WEE1, SAMSN1, MLF1, ITGB2, BCL2L11, FOXO4, NOXA1, SSH1, ARHGEF2, BAX, RAF1, TP53, ABL1, AANAT, AKT1, GAB2, TLK2, and DAPK2.
Biology Area: Cell Biology, Signal Transduction
Antigen References: 1. Funami K, et al. 2016. Mol. Immunol. 73:60. 2. Bonet R, et al. 2013. J. Mol. Biol. 425:3060. 3. Hong HY, et al. 2010. Mol. Cells 29:305. 4. Danes CG, et al. 2008. Cancer Res. 68:1760. 5. Niemantsverdriet M, et al. 2008. Oncogene. 27:1315. 6. Bialkowska K, et al. 2003. J. Biol. Chem. 278:33342.
Gene ID: 7534
UniProt: View information about 14-3-3 zeta/delta on UniProt.org
Clone: W15121A
Regulatory Status: RUO
Other Names: Protein kinase C inhibitor protein 1, KCIP-1, HEL4, YWHAD, HEL-S-3, HEL-S-93, 14-3-3-zeta
Isotype: Rat IgG1, κ