Biolegend, 688402, Purified anti-IKBKB (IKKβ) Antibody, 100μg

NF-kappa-B (NF-κB) signaling pathway is involved in many biological responses such as immune response, growth control, DNA damage and other cellular stresses. This pathway is regulated by the inhibitory IKK proteins (composed by IKKα, IKBKB/IKKβ and IKKγ) which complex with NF-κB/Rel transcription factors in the cytosol. As part of the IKK complex, IKBKB phosphorylates inhibitors of NF-κB on 2 serine residues. These phosphorylations cause polyubiquitination and subsequent degradation of the NF-κB inhibitors. In turn, free NK-κB translocates into nucleus and activates the downstream gene transcription. Upon cytokine stimulation, phosphorylation of Ser-177 and -181 enhances IKBKB activity; on the other hand, acetylation of Thr-180 residue prevents IKKβ phosphorylation and activation, thus blocking the inhibition of NF-κB pathway.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial human IKBKB recombinant protein (520-745 a.a.) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedKO/KD-WB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2617026 (BioLegend Cat. No. 688402)
Structure: 756 amino acids with a predicted molecular weight of 86.6 kD. Contains a N-terminal kinase domain and a leucine zipper region responsible for dimerization.
Distribution: Nucleus and cytoplasm.
Function: IKBKB is a serine kinase that participates in the NF-κB signaling pathway. It is activated by a variety of stimuli, including inflammatory cytokines, pathogen infections, and other cellular stresses.
Interaction: Component of the IKK core complex. Interacts with NALP2, TICAM1, FAF1, ATM, NIBP, ARRB1, ARRB2, TRIM21, NLRC5, PDPK1, EIF2AK2/PKR, ZNF268, IKBKE, NAA10, and FOXO3.
Biology Area: Cell Biology, Signal Transduction
Molecular Family: Protein Kinases/Phosphatase
Antigen References: 1. Sasaki Y, et al. 2016. Curr. Top. Microbiol. Immunol. 393:177. 2. Nielsen C, et al. 2014. J. Clin. Immunol. 34:916. 3. Amaya M, et al. 2014. Pathog. Dis. 72:32. 4. Hinz M, et al. 2014. EMBO Rep. 15:46. 5. Mousallem T, et al. 2014. Blood. 124:2046. 6. Schmid JA, et al. 2008. Cytokine Growth Factor Rev. 19:157.
Gene ID: 3551
UniProt: View information about IKBKB on UniProt.org
Clone: W15160A
Regulatory Status: RUO
Other Names: Inhibitor of nuclear factor kappa-B kinase subunit beta, IKK“Ä’, IKKbeta, NFKBIKB, IKKB, IKK2
Isotype: Mouse IgG1, κ