Biolegend, 688602, Purified anti-CaMKII Antibody, 100μg

The Ca2 + /calmodulin (CaM)-dependent protein kinases (CaMKs) are multifunctional serine/threonine kinases whose activity are regulated through Ca2 + signaling. CaMKII is expressed as a multimeric protein, which typically is comprised of 12 subunits in physiological conditions. CaMKII is an important member of the calcium/calmodulin-activated protein kinase family. It phosphorylates nearly 40 different proteins, including enzymes, ion channels, kinases, and transcription factors. CaMKII plays critical roles in neural synaptic stimulation and T cell receptor signaling. Recent studies suggested that high levels of CaMKII is also expressed in variety of cancers. It regulates proliferation, differentiation and survival of cancer cells.
100μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: 50% pure type II CaM kinase, purified through Calmodulin-Sepharose step.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedIHC-F - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg/ml. For immunohistochemical staining on frozen tisse sections, a concentration range of 2.0 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Erondu NE, et al. 1985. J. Neurosci. 5:3270.
RRID: AB_2617027 (BioLegend Cat. No. 688602)
Structure: 478 amino acids with a predicted molecular weight of 50 kD.
Distribution: Synapse and cytosol.
Function: A prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release; member of the NMDAR signaling complex in excitatory synapses it regulates NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.
Interaction: BAALC, MPDZ, SYN1, CAMK2N2 and SYNGAP1.
Biology Area: Cell Biology, Immunology, Signal Transduction
Molecular Family: Protein Kinases/Phosphatase, TCRs
Antigen References: 1. Hook SS, et al. 2001. Annu. Rev. Pharmacol. Toxicol. 41:471. 2. Hoelz A, et al. 2003. Mol. Cell 11:1241. 3. Lisman J, et al. 2012. Nat. Rev. Neurosci. 13:169. 4. Barria A, et al. 1997. Science 276:2042. 5. Hughes K, et al. 2001. J. Biol. Chem. 276:36008. 6. Wang YY, et al. 2015. Oncotarget. 6:11725.
Gene ID: 815
UniProt: View information about CaMKII on UniProt.org
Clone: 6G9
Regulatory Status: RUO
Other Names: CAMK2, CaM kinase II, Calcium/calmodulin-dependent protein kinase type II subunit alpha
Isotype: Mouse IgG1, κ