Biolegend, 690101, Purified anti-STAT6 Phospho (Tyr641) Antibody, 25μg

STAT6 is a member of the signal transducer and activator of transcription (STAT) family, activating gene expression in response to IL-4 and IL-13 stimulation. Upon cytokine stimulation, the receptor is phosphorylated by the associated Janus Kinases (Jak), followed by recruiting cytoplasmic STAT6. The Tyr641 residue of STAT6 is, in turn, phosphorylated by Jak. Phosphorylated STAT6 forms homodimers, translocates to the nucleus, and regulates transcription of target genes. STAT6 plays crucial roles in differentiation of T helper 2 (Th2) cells, class switch of immunoglobulins in B cells, expression of cell surface markers such as MHC class II, and the development of allergic inflammation.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human STAT6 peptide phosphorylated at Tyr641.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, ChIP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.01 - 0.05 µg per ml. For immunocytochemistry, a concentration range of 0.2 - 1.0 μg/ml is recommended. For ChIP applications, the suggested dilution is 1:50-1:100 by volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody recognizes STAT6 Phospho (Tyr641) in all three isoforms at 74, 82, and 94 kD. The predominant band detected is at 94 kD.
RRID: AB_2715760 (BioLegend Cat. No. 690101) AB_2715760 (BioLegend Cat. No. 690102)
Structure: 847 amino acids, predicted molecular weight of 94 kD. Contains a SH2 domain responsible for phosphor-Tyrosine binding and dimerization.
Distribution: Translocates to the nucleus when phosphorylated.
Function: STAT6 is a transcription factor, mediating IL-4 and IL-13 signaling.
Interaction: STAT6 interacts with CBP/p300 and NCOA1. Forms a homodimer or a heterodimer with other STAT family members.
Cell Type: B cells
Biology Area: Cell Biology, Immunology, Signal Transduction
Molecular Family: Nuclear Markers, Phospho-Proteins
Antigen References: Goenka S, et al. 2011. Immunol. Res. 50:87 Wurster AL, et al. 2000. Oncogene. 19:2577 Akira S, 1999. Stem Cells. 17:138 Zamorano J, et al. 2005. J. Immunol. 174:2843 David M, et al. 2001. Oncogene. 20:6660 Takeda K, et al. 1996. Nature. 380:627
Gene ID: 6778
UniProt: View information about STAT6 Phospho Tyr641 on UniProt.org
Clone: A15137A
Regulatory Status: RUO
Other Names: Signal transducer and activator of transcription 6, IL-4 Stat
Isotype: Mouse IgG1, κ