Biolegend, 692602, Purified anti-HGF Antibody, 100μg

HGF (Hepatocyte growth factor) is a multifunctional cytokine that plays important roles in cell proliferation, survival, motility and morphogenesis. HGF protects the epithelium, neurons and cardiomyocytes during organ diseases; this protective effect is mediated through anti-apoptotic signals via inhibition of caspase-3 activity or induction of anti-apoptotic molecules. Secreted by cells of mesenchymal origin, HGF is the specific ligand for the tyrosine-kinase receptor c-MET (cellular mesenchymal-epithelial transition), also called MET, which is expressed in different types of epithelial, endothelial and hematopoietic progenitor cells. Aberrantly activated MET/HGF signaling correlates with tumorigenesis and metastasis, and is regarded as a target for novel anti-cancer treatments.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant Human HGF (carrier-free)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody works for Western blotting under non-reducing conditions only and does not cross-react with mouse.
RRID: AB_2632766 (BioLegend Cat. No. 692602)
Structure: Heterodimer (alpha and beta chains). Pro-HGF has 728 amino acids, the predicted molecular weight is approximately 83 kD. The alpha and beta chains contain 463 and 234 amino acids and have a predicted molecular mass of approximately 53.6 and 26 kD respec
Distribution: Mesenchymal cells, liver sinusoidal endothelial, and macrophages.
Function: Stimulates epithelial cell proliferation, motility, morphogenesis and angiogenesis. Hypoxia and hyperglycemia suppress HGF production. Also, TGF-β and angiotensin-II depress HGF production in vitro.
Interaction: Hepathocytes and epithelial cells.
Ligand/Receptor: MET receptor.
Biology Area: Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Cell Proliferation and Viability, Immunology, Signal Transduction
Molecular Family: Cytokines/Chemokines, Growth Factors
Antigen References: 1. Hartmann S, et al. 2016. Clin. Cancer Res. 22:4005. 2. Fajardo-Puerta AB, et al. 2016. J. Clin. Pathol. 69:575. 3. Zhang Y, et al. 2016. Oncotarget. 7:37370-89. 4. Frisch RN, et al. 2016. Expert Opin. Ther. Targets 20:1087. 5. Kataoka H, et al. 2000. J. Biol. Chem. 275:40453. 6. Wang X, et al. 2002. Mol. Cell. 9:411. 7. Wu MH, et al. 2004. Gene Ther. 11:170. 8. Mizuno S, et al. 2008. Front. Biosci. 13:7072. 9. D'Angelo F, et al. 2013. Clin. Exp. Immunol. 174:60.
Gene ID: 3082
UniProt: View information about HGF on UniProt.org
Clone: A15061E
Regulatory Status: RUO
Other Names: Hepatocyte Growth Factor, Scatter Factor (SF), Hepatopoietin (HPTA), Lung fibroblast-derived mitogen
Isotype: Mouse IgG2b, κ