Biolegend, 693402, Purified anti-HDAC5 Antibody, 100μg

Histone deacetylases (HDACs) are a family of enzymes capable of regulating acetylation level of histones and non-histone proteins. HDACs have been classified into four classes (class I, II, III, and IV) based on their homology with yeast HDACs. HDAC5 is a member of class IIa HDACs, which contain N-terminal extensions with 14-3-3 binding sites. Phosphorylation of HDAC5 on Ser259 and Ser498 leads to association with 14-3-3 proteins and export from the nucleus. HDAC5 has been suggested to participate in various cellular activities, such as cell proliferation, cell cycle progression, apoptosis, neuronal repair, synoviocyte activation, and myoblast differentiation. Aberrant expression of HDAC5 has been shown to be associated with tumor progression and poor prognosis in cancer patients.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial human HDAC5 recombinant protein (320-480 a.a.) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2876744 (BioLegend Cat. No. 693401) AB_2632835 (BioLegend Cat. No. 693402)
Structure: 1122 amino acids with a predicted molecular weight of 122 kD and observed of approximately 140 kD. Contains a histone deacetylation domain responsible for the removal of acetyl group on the side chain of lysine residue.
Distribution: Nucleus and cytoplasm.
Function: HDAC5 is a class II histone deacetylase and plays important roles in cell proliferation, differentiation, apoptosis, and tumor progression.
Interaction: Interacts with AHRR, BAHD1, BCOR, HDAC7, HDAC9, CTBP1, MEF2C, NCOR2, NRIP1, PHB2, 14-3-3, BCL6, DDIT3/CHOP, GRK5, KDM5B, MYOCD, and EP300.
Biology Area: Cell Biology, Chromatin Remodeling/Epigenetics
Antigen References: 1. Xiao H, et al. 2016. Int. J. Cancer 138:2477. 2. Chen S, et al. 2015. Cell Cycle 14:2520. 3. Cho Y, et al. 2013. Cell. 155:894. 4. Kim SS, et al. 2013. Mol. Cell. 52:289. 5. Cho Y, et al. 2012. EMBO J. 31:3063. 6. Watamoto K, et al. 2003. Oncogene. 22:9176.
Gene ID: 10014
UniProt: View information about HDAC5 on UniProt.org
Clone: W15202A
Regulatory Status: RUO
Other Names: Histone deacetylase 5 (HD5), Antigen NY-CO-9, KIAA0600
Isotype: Rat IgG2b, κ