Biolegend, 694402, Purified anti-human CCL22 Antibody, 100μg

CCL22 is thymus-specific chemokine engaged in the recruitment of T cells. CCL22 is associated with different diseases such as allergen-induced lung inflammation, atopic dermatitis, and lymphoma. Also, high levels of CCL22 have been detected in the CNS in multiple sclerosis (MS) and experimental autoimmune encephalomyelitis (EAE). In addition, CCR4+ T cells have been linked to endotoxic shock, rheumatoid arthritis, T cell lymphoma, and autoimmune diabetes. CCL22 is a prostaglandin-dependent pyrogen, acting in the anterior hypothalamus to induce hyperthermia via activation of brown adipose tissue.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human CCL22
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: Direct ELISA - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.
Application Notes: Clones A15083A and A15083D do not block each other Clone A15083A does not block the reference clone T51-719 (BD Bioscience) Clones A15083A and A15083D showed good staining in both Cyto-Fast™ Fix/Perm Buffer Set and True-Phos™ Perm Buffer.
Additional Product Notes: This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone A15083D) could function as a detection antibody when paired with purified anti-human CCL22 (MDC) antibody (Cat. No. 611952) (clone A15083A) as the capture antibody and using Recombinant Human CCL22 (MDC) protein (Cat. Nos. 584902 and 584904) as the assay standard. For assay development flexibility purpose this product is provided as purified format.
RRID: AB_2941631 (BioLegend Cat. No. 694402)
Structure: Chemokine
Distribution: Macrophages, NK cells, IFN-γ stimulated human keratinocytes, thymic epithelial cells, and intestinal epithelial cells
Function: CCL22 induces the migration of CCR4+ Th2 cells, Tregs, DC, and NK cells. It is induced by LPS and CD40 antibody in macrophages. It is upregulated by Th2 cytokines (IL-4 and IL-5) and downregulated by Th1 cytokines (IFN-γ).
Interaction: Dendritic cells, NK cells, Th2 cells, T regs, CLA+ skin-homing memory/effector T cells
Ligand/Receptor: CCR4
Biology Area: Cell Biology, Signal Transduction
Molecular Family: Cytokines/Chemokines
Antigen References: Godiska R, et al. 1997. J Exp Med. 185:1595-604. Imai T, et al. 1998. J Biol Chem. 273:16551-4. Yamashita U and Kuroda E. 2002. Crit Rev Immunol. 22:105-14. Horikawa T, et al. 2002. Int Immunol. 14:767-73. Mailloux AW and Young MR. 2009. J Immunol. 182:2753-65. Dogan RN, et al. 2011. J Leukoc Biol. 89:93-104. Osborn O, et al. 2011. Cytokine. 53:311-9.
Gene ID: 6367
UniProt: View information about CCL22 on UniProt.org
Clone: A15083D
Regulatory Status: RUO
Other Names: Macrophage derived cytokine (MDC), Small inducible cytokine subfamily A, member 22 (Scya22), stimulated T cell chemotactic protein (STCP-1)
Isotype: Mouse IgG2a, κ