Biolegend, 694407, PE anti-human CCL22 Antibody, 25tests

CCL22 is thymus-specific chemokine engaged in the recruitment of T cells. CCL22 is associated with different diseases such as allergen-induced lung inflammation, atopic dermatitis, and lymphoma. Also, high levels of CCL22 have been detected in the CNS in multiple sclerosis (MS) and experimental autoimmune encephalomyelitis (EAE). In addition, CCR4+ T cells have been linked to endotoxic shock, rheumatoid arthritis, T cell lymphoma, and autoimmune diabetes. CCL22 is a prostaglandin-dependent pyrogen, acting in the anterior hypothalamus to induce hyperthermia via activation of brown adipose tissue.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human CCL22
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clones A15083A and A15083D do not block each other Clone A15083A does not block the reference clone T51-719 (BD Bioscience) Clones A15083A and A15083D showed good staining in both Cyto-Fast™ Fix/Perm Buffer Set and True-Phos™ Perm Buffer.
RRID: AB_3068170 (BioLegend Cat. No. 694407) AB_3068170 (BioLegend Cat. No. 694408)
Structure: Chemokine
Distribution: Macrophages, NK cells, IFN-γ stimulated human keratinocytes, thymic epithelial cells, and intestinal epithelial cells
Function: CCL22 induces the migration of CCR4+ Th2 cells, Tregs, DC, and NK cells. It is induced by LPS and CD40 antibody in macrophages. It is upregulated by Th2 cytokines (IL-4 and IL-5) and downregulated by Th1 cytokines (IFN-γ).
Interaction: Dendritic cells, NK cells, Th2 cells, T regs, CLA+ skin-homing memory/effector T cells
Ligand/Receptor: CCR4
Biology Area: Cell Biology, Signal Transduction
Molecular Family: Cytokines/Chemokines
Antigen References: Godiska R, et al. 1997. J Exp Med. 185:1595-604. Imai T, et al. 1998. J Biol Chem. 273:16551-4. Yamashita U and Kuroda E. 2002. Crit Rev Immunol. 22:105-14. Horikawa T, et al. 2002. Int Immunol. 14:767-73. Mailloux AW and Young MR. 2009. J Immunol. 182:2753-65. Dogan RN, et al. 2011. J Leukoc Biol. 89:93-104. Osborn O, et al. 2011. Cytokine. 53:311-9.
Gene ID: 6367
UniProt: View information about CCL22 on UniProt.org
Clone: A15083D
Regulatory Status: RUO
Other Names: Macrophage derived cytokine (MDC), Small inducible cytokine subfamily A, member 22 (Scya22), stimulated T cell chemotactic protein (STCP-1)
Isotype: Mouse IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.