Biolegend, 694802, Purified anti-human FOXP3 Delta 2 (exon 2 deleted) Antibody, 100μg

FOXP3 is a transcription factor which is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4 + /CD25 - cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in "scurfy" mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity. In human, unlike in mouse, two isoforms of FOXP3 have been reported: one (FOXP3) corresponding to the canonical full-length sequence; the other (FOXP3 delta 2) lacking exon 2. This antibody reacts to human FOXP3 delta 2 (exon 2 deleted) form and weakly reacted to the full-length FOXP3.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human FOXP3 delta2 (exon 2 deleted) peptide conjugated to KLH.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody is against human FOXP3 delta 2, molecular weight of 43 kD, and weakly reacts with full length human FOXP3, molecular weight 47 kD.
RRID: AB_2650734 (BioLegend Cat. No. 694802)
Structure: Forkhead/winged-helix transcription factor family, exon 2 deleted, approximately 43 kD, contains zinc finger and forkhead domains
Distribution: Nuclear; expressed in T regulatory cells.
Function: Transcription factor proposed to be a master regulatory gene in T regulatory cell development and a critical factor for immune homeostasis.
Interaction: IKZF3, STUB1, HSPA8 and HSPA1A/B, PPP1CA, PPP1CB, PPP1CG, KAT5, HDAC7, HDAC9, USP7, RUNX1, RORC, RUNX2, RUNX3 and IKZF4.
Cell Type: Tregs
Biology Area: Cell Biology, Cell Proliferation and Viability, Chromatin Remodeling/Epigenetics, Immunology, Innate Immunity, Signal Transduction, Transcription Factors, Ubiquitin/Protein Degradation
Antigen References: 1. Kristensen B, et al. 2015. Clin. Exp. Immunol. 180:58. 2. Roncador G, et al. 2005. Eur. J. Immunol. 35:1681. 3. Mayack S, et al. 2006. J. Immunol. 176:2059. 4. Yang ZZ, et al. 2006. Blood 107:3639. 5. Gavin MA, et al. 2006. Proc. Natl. Acad. Sci. USA 103:6659. 6. Groh V, et al. 2006. Nature Immunology 7:755. 7. Lewkowicz P, et al. 2006. J. Immunol. 177:7155. 8. Luke PP, et al. 2006. Am. J. Transplant. 6(9):2023. 9. Bamias G, et al. 2007. J. Immunol. 178:1809. 10. Valencia X, et al. 2007. J. Immunol. 178:2579. 11. Davidson TS, et al. 2007. J. Immunol. 178:4022.
Gene ID: 50943
UniProt: View information about FOXP3 on UniProt.org
Clone: A15080A
Regulatory Status: RUO
Other Names: Forkhead box protein P3, Scurfin, JM2, IPEX, Zinc finger protein JM2
Isotype: Mouse IgG2a, κ
Q: Can FOXP3 be costained with cytokines?
A: The larger holes created by the nuclear permeabilization required for FOXP3 may allow cytokines to leak out of the cell, making it harder to detect lowly-expressed cytokines. You may have to use a control where the cells are only permeabilized through the cell membrane.