Biolegend, 695902, Purified anti-NF-κB p100/p52 Antibody, 100μg

Nuclear factor NF-kappa-B p100/p52 subunit (NF-κB2) is a member of the Rel/NF-κB family of transcription factors. NF-κB2 p100 subunit contains an ankyrin repeat domain that has IκB activity to retain NF-κB2 proteins in the cytoplasm. Upon activation, NF-κB2 p100 subunit is processed to p52 subunit through the ubiquitin-proteasome pathway and translocated to the nucleus. NF-κB2 p52 subunit forms homo- or heterodimers with other Rel-like domain-containing proteins, including c-REL, RELA/p65, RELB, NFKB1/p105, and NFKB1/p50, subsequently binds to specific kappa-B sites and regulates expression of their target genes. NF-κB participates in the regulation of peripheral lymphoid development, thymic development, and B cell maturation. Mutations in the NF-κB2 gene lead to defects in B cell development and have been associated with lymphoma and other hematogenous malignancies.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Human NF-κB2 recombinant protein (1-250 a.a.) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/ml. For immunocytochemistry, a concentration range of 0.2 - 1.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone W16082A does not cross-react with mouse (in-house tested).
RRID: AB_2650935 (BioLegend Cat. No. 695902)
Structure: NF-κB2 p100 subunit contains 900 amino acids with a predicted molecular weight of 96.7 kD. Contains an N-terminal RHD domain for DNA binding and a C-terminal ankyrin repeat domain. NF-κB2 p100 subunit is processed to a p52 subunit upon activation.
Distribution: Nucleus and cytoplasm.
Function: NF-κB2 is a transcription factor involved in the regulation of peripheral lymphoid development, thymic development, and B cell maturation.
Interaction: Forms homodimers or heterodimers with other Rel-like domain-containing proteins including c-REL, RELA/p65, RELB, NFKB1/p105, and NFκB1/p50. Interacts with BCL3, BTRC, TSC22D3, and MAP3K8.
Biology Area: Cell Biology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Lee CE, et al. 2014. Blood. 124:2964. 2. Savinova OV, et al. 2009. Mol Cell. 34:591. 3. Tucker E, et al. 2007. J. Immunol. 179:7514. 4. Kim KE, et al. 2000. Oncogene. 19:1334. 5. Heusch M, et al. 1999. Oncogene. 18:6201. 6. de Wit H, et al. 1998. Leukemia. 12:363.
Gene ID: 4791
UniProt: View information about NF-kappaB p100/p52 on UniProt.org
Clone: W16082A
Regulatory Status: RUO
Other Names: DNA-binding factor KBF2, H2TF1, Lymphocyte translocation chromosome 10 protein, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2, Oncogene Lyt-10
Isotype: Rat IgG2b, κ