Biolegend, 696004, Ultra-LEAF™ Purified anti-human CRP (C-reactive protein) Antibody, 1mg

C-reactive protein (also known as CRP and Pentraxin 1 (PTX1)), is a member of pentraxin protein family. Similar to other pentraxins, CRP is a pattern recognition molecule that is able to recognize pathogens and promote their clearance. CRP tends to form a non-covalently linked pentamer with each subunit made up of two antiparallel β-sheets and a single short α-helix. It is characterized by the presence of a cleft that extends from the center of the subunit to its edge at the central pore of the pentamer structure. It has been demonstrated that CRP binds to the phosphocholine moieties expressed on the surface of dead or dying cells and some bacteria. This leads to the activation of the complement system and promotion of phagocytosis by macrophages to clear necrotic and apoptotic cells. CRP is secreted by hepatocytes and is a biomarker for inflammation. IL-6 has been shown to be an effective inducer of this protein. Besides binding to apoptotic cells, CRP also binds to a variety of molecules including C1q, bacterial polysaccharide, CD64, CD32, APCS, HDL, and fibronectin. In addition to participating in immune responses, CRP is also a prominent partaker in endothelial dysfunction and atherosclerosis. It was shown that CRP may exhibit direct proantherogenic effects by upregulating angiotensin type I receptor in smooth muscle cells and promoting their migration, proliferation, neointimal formation, and reactive oxygen species production. Mature human CRP shares 71% amino acid sequence homology with its mouse counterpart.
1mg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human CRP/PTX1
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: Neut - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by blocking the binding of recombinant human APCS (1 µg/mL) to immobilized recombinant human CRP (2 µg/mL). ND50 range: 0.4 - 2.0 mg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2686982 (BioLegend Cat. No. 696003) AB_2686983 (BioLegend Cat. No. 696004)
Structure: Monomer and homopentamer
Distribution: Hepatocytes
Function: Induces expression of E-selectin and VCAM-1 expression in epithelial cells. CRP binds to target, initiates, and regulates complement activation. Induced by IL-1 and IL-6.
Interaction: Phosphocholine on the surface of apoptotic cells
Ligand/Receptor: Interacts with C1q, bacterial polysaccharide, CD64, CD32, APCS (PTX2), HDL, leptin, and fibronectin.
Biology Area: Cell Biology
Antigen References: 1. Ansar W, Ghosh S. 2013. Immunol. Res. 56:131. 2. Agrawal A, et al. 2009. Adv. Exp. Med. Biol. 653:98. 3. Garlanda C, et al. 2005. Annu. Rev. Immunol. 23:337. 4. Verma S, et al. 2004. Circulation 109:1914. 5. Wadham C, et al. 2004. Circulation 109:2116. 6. Li XA, et al. 1998. Biochem. Biophys. Res. Commun. 244:249. 7. Lu J, et al. 2012. Immunol. Rev. 250:230. 8. Chen K, et al. 2006. Nat. Med. 12:425.
Gene ID: 1401
UniProt: View information about CRP on UniProt.org
Clone: A16065C
Regulatory Status: RUO
Other Names: C-reactive protein (CRP), Pentraxin 1 (PTX1)
Isotype: Mouse IgG1, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.