Biolegend, 696702, Purified anti-Tapasin (Tapbp) Antibody, 100μg

Major histocompatibility complex (MHC) class I molecules present peptide antigens recognized by CD8 + T cells to enable the immune recognition of virally or malignantly transformed cells exposing non-self peptides. Selection and loading of peptides onto MHC I is orchestrated by the peptide-loading complex (PLC). Tapasin, a central component of the PLC, stabilizes MHC I and catalyzes the exchange of low-affinity against high-affinity, immunodominant peptides. In this quality-control step, tapasin cooperates with other more general, ER-resident chaperones, including ERp57 and TAP. Tapasin has been found to be downregulated in glioblastoma, renal cell carcinoma, prostate cancer, urothelial carcinoma, cervical carcinoma, head and neck carcinoma and esophageal squamous cell carcinoma. Studies also reported reduced tapasin to be linked to metastasis and poor outcome.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: Partial mouse tapasin peptide (11-34 a.a.)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone does not cross-reacts with human (in-house tested). This antibody recognizes a non-specific band over 100 kD in some tested cell lysates.
Product Citations: Boulanger DSM, et al. 2022. Front Immunol. 13:956603. PubMed Deng J, et al. 2021. Nat Cancer. 2:503. PubMed
RRID: AB_2687096 (BioLegend Cat. No. 696702)
Structure: 465 amino acids with a predicted molecular weight of 50 kD. An isoform with predicted MW of 47kD was reported.
Distribution: Endoplasmic reticulum membrane
Function: Involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide.
Interaction: Interacts with TAP1 and is thus a subunit of the TAP complex. Obligatory mediator for the interaction between newly assembled MHC class I molecules, calreticulin, ERp57 and TAP.
Cell Type: Embryonic Stem Cells
Biology Area: Cell Biology, Immunology, Stem Cells, Ubiquitin/Protein Degradation
Molecular Family: MHC Antigens
Antigen References: Neefjes J, et al. 2011. Nat. Rev. Immunol. 11:823-36. Dong G, et al. 2009. Immunity. 30:21. Fisette O, et al. 2016. Sci. Rep. 6:19085. Dick TP, et al. 2002. Immunity. 16: 87. Ortmann B, et al. 1997. Science. 277: 1306. Thuring C, et al. 2015. Br. J. Cancer. 113:952. Han LY, et al. 2008. Clin. Cancer Res. 14: 3372. Seliger B, et al. 2010. Cancer Immunol. Immunother. 59: 529.
Gene ID: 21356
UniProt: View information about Tapasin on UniProt.org
Clone: 5D3/Tapasin
Regulatory Status: RUO
Other Names: TPN, TPSN, TAP-associated protein, Tapbp, Tapa
Isotype: Armenian Hamster IgG