Biolegend, 698406, Alexa Fluor® 488 anti-Histone H3 Acetylated Lysine 9 (K9ac) Antibody, 100μg

Histone subunit H3, along with subunits 2A, 2B, and 4, make up the core subunits of the nucleosome octomer. An octomer contains two protomers of each subunit tightly wrapped around a ~147 bp segment of DNA. Histones have integral roles in chromatin integrity, genomic stability, and gene regulation. Post-translational modification of histones in response to certain stimuli results in alterations of nucleosomal positioning relative to DNA.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Histone H3 K9ac peptide
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2.5 - 5 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Application Notes: This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).
RRID: AB_2832850 (BioLegend Cat. No. 698405) AB_2832851 (BioLegend Cat. No. 698406)
Structure: Histone H3 is a 134 aa protein with a predicted molecular weight of 15.4 kD. The subunit is comprised of a histone fold, an αN-helix main globular domain and a large N-terminal tail harboring residues subject to multiple types of post-translational.
Distribution: Nucleus
Function: Histone acetylation is generally associated with activation of gene expression, nucleosomal redistribution, and histone deposition. One consequence of this modification is the alteration of chromatin structure to facilitate recruitment of transcription factors. Additionally, acetyl-lysine modification is also used as binding site for proteins containing bromodomains, resulting in their recruitment to the modified histones.
Interaction: Histones 2A, 2B, 4, HDAC
Biology Area: Cell Biology, Chromatin Remodeling/Epigenetics
Antigen References: 1. Zhang, et al. 2017. Nat. Commun. 8: 14799. 2. Kanda M, et al. 2016. Proc. Natl. Acad. Sci. USA 113: E3394. 3. Bellet MM, et al. 2013. Proc. Natl. Acad. Sci. USA 9: 3333. 4. Li B, et al. 2016. Sci. Rep. 6: 26542. 5. Li D, et al. 2014, Mol. Cancer. 13:26.
Gene ID: 8350
UniProt: View information about Histone H3 on UniProt.org
Clone: 2G1F9
Regulatory Status: RUO
Isotype: Rat IgG2a, κ