Biolegend, 699402, Purified anti-Calnexin Antibody, 100μg

Calnexin is a type I integral ER transmembrane chaperone that interacts with the oligosaccharide Glc1Man9GlcNAc2 on the glycoproteins and possesses binding sites for ATP and calcium.  Together with its soluble homolog calreticulin and the thiol oxidoreductase ERp57, calnexin was found to participate in the folding and quality control of newly synthesized N-linked glycoproteins. Calnexin has calcium-binding capacity and is involved in the regulation of calcium homeostasis. The potential calnexin binding substrates include MHC class I molecules, ion channel proteins, and the cystic fibrosis transmembrane conductance regulator (CFTR). Mutations on calnexin have been implicated in several coagulation disorders and cystic fibrosis. Calnexin-deficient mice exhibit severe motor problems and growth defects.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Human Calnexin recombinant protein (161-410 a.a.) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml (1:500-1:5000 diluted). For immunocytochemistry, a concentration range of 0.5 - 2.0 μg/ml (1:250-1:1000 dilution) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The apparent molecular weight of Calnexin may differ based on the gel system used: in Tris-Glycine gels, a molecular weight of ~90 kDa was observed by WB testing, while in Bis-Tris gels an apparent molecular weight of ~75 kDa was observed.
Product Citations: Hugendieck G, et al. 2023. J Extracell Vesicles. e12338:12. PubMed Tábara LC, et al. 2022. Brain. 145:3095. PubMed Antonicka H, et al. 2020. Cell Metabolism. 32(3):479-497.e9. PubMed Zhang Y, et al. 2021. J Extracell Vesicles. e12073:10. PubMed Aaltonen MJ, et al. 2022. Life Sci Alliance. 5:. PubMed Kiianitsa K, et al. 2021. J Leukoc Biol. 110:829. PubMed
RRID: AB_2728519 (BioLegend Cat. No. 699401) AB_2728520 (BioLegend Cat. No. 699402)
Structure: 592 amino acids with a predicted molecular weight of 67.6 kD. Type I transmembrane protein. Contains an N-terminal ER lumenal domain and a C-terminal cytoplasmic domain.
Distribution: Endoplasmic reticulum membrane.
Function: Calnexin is an ER transmembrane chaperone protein and functions in the folding of newly synthesized glycoprotein and quality control of misfolded proteins.
Interaction: Interacts with MAPK3/ERK1, KCNH2, SGIP1, SERPINA2P/SERPINA2, PPIB, and ZNRF4.
Biology Area: Cell Biology
Antigen References: Lamriben L, et al. 2016. Traffic. 17:308. Kuang XL, et al. 2014. J. Neurosci. Res. 92:1319. Lederkremer GZ. 2009. Curr. Opin. Struct. Biol. 515-23. Beauregard PB, et al. 2009. J. Cell Sci. 122:1342. Rosser MF, et al. 2008. Mol. Biol. Cell. 4570-9. Caramelo JJ, Parodi AJ. 2008. J. Biol. Chem. 283:10221.
Gene ID: 821
UniProt: View information about Calnexin on UniProt.org
Clone: W17077C
Regulatory Status: RUO
Other Names: CANX, IP90, Major histocompatibility complex class I antigen-binding protein p88, p90
Isotype: Rat IgG2a, κ