Biolegend, 699501, Purified anti-p63 (ΔN) Antibody, 25μg

p63 (ΔN) is a member of the p53 family. This protein has multiple isoforms ΔN p63-α, -β, and -γ. The molecular weights of the p63 isoforms are: α=66 kD, β=52 kD, and γ=45 kD. This nuclear protein is essential for limb formation, carcinogenesis, epidermal morphogenesis, and epithelial stem cell regeneration. ΔN isoforms are antagonistic to p53; TA isoforms can transactivate p53 targets. The p63 ΔN isoforms repress TA isoforms; mutant p53 abrogates p63 transactivation. This protein interacts with p53, p73, and the various TA- and ΔN isoforms.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Synthetic peptide
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg/mL (1:250 - 1:1000 dilution). For immunocytochemistry, a concentration range of 2.0 - 5.0 μg/mL (1:100 - 1:250 dilution) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody does not recognize TA isoforms of p63. Due to complete sequence homology between the immunizing peptide and mouse p63 (ΔN), this clone is predicted to recognize mouse p63 (ΔN) isoforms.
Product Citations: Zhong Z, et al. 2022. Bio Protoc. 12: . PubMed Chalmers FE, et al. 2022. Dev Biol. 485:9. PubMed
RRID: AB_2820119 (BioLegend Cat. No. 699501) AB_2820120 (BioLegend Cat. No. 699502)
Structure: p53 family, six isoforms, ΔN p63α, β, γ, approximately 66 kD, 52 kD, 45 kD
Distribution: Nuclear
Function: Essential for limb formation, epidermal morphogenesis, epithelial stem cell regeneration. ΔN isoforms are antagonistic to p53.
Interaction: p53, p73, TA- and ΔN isoforms
Biology Area: Cell Biology, Transcription Factors
Molecular Family: Tumor Suppressors
Antigen References: 1. Yang A, et al. 1998. Mol Cell. 2:305. 2. Celli J, et al. 1999. Cell. 99:143. 3. Gaiddon C, et al. 2001. Mol Cell Biol. 21:1874. 4. Benard J, et al. 2003. Hum Mutat. 21:182. 5. Mangiulli M, et al. 2009. Nucleic Acids Res. 37:6092
Gene ID: 8626
UniProt: View information about p63 on UniProt.org
Clone: W17048F
Regulatory Status: RUO
Other Names: p63, ΔN p63-α, p63-β, p63-γ, p63-δ, TP63, tumor protein p63
Isotype: Rat IgG2a, κ