Biolegend, 699907, , 1kit

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. α subunit plays an important role in subunit assembly since its dimerization is the first step in the sequential assembly of subunits to form the holoenzyme. The β subunit is the second-largest subunit, and is encoded by the rpoB gene. The β’ (β prime) subunit contains structures crucial for transcription, including the sites for ribonucleotide addition and catalysis. For additional antigen details, please refer to individual product datasheets.
1kit
Formulation: Please refer to individual product datasheets for details.
Preparation: All antibodies in this kit were purified by affinity chromatography.
Storage & Handling: Upon receipt, store undiluted at 2-8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of antibodies in this kit is quality control tested by Western Blotting. For Western blotting, the suggested uses of these reagents are as follows: Anti-E. coli RNA Polymerase β Prime (clone NT73): 1:1000-1:4000 (0.25-1ug/mL) Anti-E. coli RNA Polymerase β (clone NT63): 1:2500-1:100000 (0.005-0.2ug/mL) Anti-E. coli RNA Polymerase α (clone 4RA2): 1:500-1:5000 (0.1-1.0 ug/mL) Anti-E. coli RNA Polymerase β (clone 8RB13): 1:2500-1:100000 (0.005-0.2ug/mL) It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For verified or reported applications for these antibodies, please see individual product datasheets. Clone NT73 recognizes epitope 1295-1417 (SLAELLNAGLGGS), and is extremely useful for purifying holoenzymes from enterics. It does not cross-react with mycobacterial smegmatis RNAP. This antibody is used for purifying Softag 1 epitope tagged protein expressed in eukaryotic systems. Clone NT63 recognizes epitope 922-1090 of ß subunit of the E. coli RNA polymerase and can be used in WB analysis. Clone 8RB13 is extremely useful for detecting (WB) or purifying (IP) core RNAP from a broad range of bacteria. Clone 4RA2 specifically recognizes a subunit of the E. coli RNA polymerase.
Product Citations: Tsoumtsa Meda LL, et al. 2022. Gut Microbes. 14:2121577. PubMed
Biology Area: Cell Biology
Antigen References: Thompson NE, et al. 1992. Biochemistry 31:7003. Thompson NE, et al. 2003. Anal. Biochem. 323:171. Lesley SA and Burgess RR. 1989. Biochemistry 28:7728.
Gene ID: 948488947794
Regulatory Status: RUO
Other Names: RNA pol beta prime, rpoC, tabB, DNA-directed, RNAP subunit beta prime, Transcriptase subunit beta prime, rpoB, groN, nitB, rif, ron, stl, stv, tabD, RNA polymerase subunit alpha, Transcriptase subunit alpha, pez, phs, sez