Biolegend, 699953, PhosphoPair mTOR (Ser2448) Antibody Set, 1kit

mTOR is a member of the PI3 family of kinases and plays a critical role in regulating cell proliferation and metabolism in response to nutrient availability, cellular energy status, hormones, growth factors, and stress. mTOR is found as a component of two functionally distinct oligomeric complexes, mTORC1 and mTORC2 (mTOR complexes 1 and 2). mTORC1 coordinates nutrient availability with cell growth and proliferation through phosphorylation of upstream regulators of proliferation and ribosomal biogenesis including eIF4BP1, RPS6KB1 and RPS6KB2. Additionally, mTORC1 controls autophagy through negative regulation of an autophagy-promoting kinase complex comprised of ULK1, ATG13, and FIP200. mTORC2 regulates proliferation and metabolism via phosphorylation of IG-1R, InsR, and Akt, and also plays a role in cytoskeletal architecture and reorganization through modification of Rac1. The mTOR subunit of mTORC1 and mTORC2 is phosphorylated at Ser2448 in a PI3/Akt-signaling-dependent manner; this post-translational modification is associated with increased mTORC activity.
1kit
Verified Reactivity: Human, Mouse
Immunogen: Please refer to individual product datasheets for immunogen information
Formulation: Phosphate-buffered solutions, pH 7.2, containing 0.09% sodium azide
Preparation: The antibodies were purified by affinity chromatography.
Storage & Handling: The antibody solutions should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of antibodies in this set is quality control tested by Western blotting. For Western blotting, the suggested uses of these reagents are as follows: Clone 6H9B10: 0.25 - 1.0 µg/mL Clone A17024A: 0.1 - 1.0 µg/mL It is recommended that the reagent be titrated for optimal performance for each application. For recommended usage information for additional applications, please refer to the datasheet of the individual products.
Structure: mTOR is a 2,549 amino acid protein with a predicted molecular weight of ~289 kD.
Distribution: Ubiquitously expressed/cytosolic, lysosomal, and nuclear cellular localization
Function: Regulates cell proliferation and metabolism
Antigen References: Brown EJ, et al. 1994. Nature. 369:756-8. Dennis P, et al. 2001. Science. 294:1102. Fingar D, et al. 2002. Genes Dev. 16:1472. Nojima H, et al. 2003. J. Biol. Chem. 278:15461.
Gene ID: 2475
UniProt: View information about mTOR on UniProt.org
Clone: 6H9B10; A17024A;
Regulatory Status: RUO
Other Names: Mechanistic Target of Rapamycin Kinase, FRAP1, RAFT, FKBP-Rapamycin Associated Protein