Biolegend, 699954, PhosphoPair Lck (Tyr394) Antibody Set, 1kit

The Src family tyrosine kinase p56 Lck (Lck) is a non-receptor tyrosine kinase that plays a critical role in T cell selection and maturation within the thymus, and also in the function of mature T cells. Lck, which is constitutively bound to cytosolic domains of CD4 and CD8 surface receptors, plays an essential role in T cell receptor (TCR) signaling. Engagement of the TCR with peptide antigen-loaded MHC complexes results in the recruitment of CD4- and CD8-bound Lck to the TCR/CD3 signaling complex. Lck then transphosphorylates TCR-gamma chains and CD3 subunits, thereby activating the TCR/CD3 signaling pathway and leading to the recruitment and subsequent phosphorylation of Zap70 by Lck. Lck also plays an important role in interleukin-2 signaling that regulates the T cell proliferative response. Phosphorylation of Lck by CSK at tyrosine 505 negatively regulates the kinase, and is proposed to generate a closed, inactive conformation of the protein. Conversely, phosphorylation at tyrosine 394 in the activation loop of Lck greatly stimulates enzymatic activity. In many types of cancer, Lck is a proliferative and anti-apoptotic driver, and has been proposed as a target for therapeutic intervention.
1kit
Verified Reactivity: Human
Immunogen: Please refer to individual product datasheets for immunogen information
Formulation: Phosphate-buffered solutions, pH 7.2, containing 0.09% sodium azide
Preparation: The antibodies were purified by affinity chromatography.
Storage & Handling: The antibody solutions should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of antibodies in this set is quality control tested by Western blotting. For Western blotting, the suggested uses of these reagents are as follows: Clone LCK-01: 1.0 µg/mL Clone A18002D: 0.1 - 1.0 µg/mL It is recommended that the reagent be titrated for optimal performance for each application. For recommended usage information for additional applications, please refer to the datasheet of the individual products.
Structure: Lck is a 509 amino acid protein with a predicted molecular weight of ~58 kD.
Distribution: T cells/Plasma membrane-associated
Function: T cell receptor signaling
Antigen References: Phillipsen L, et al. 2017. Sci Signal. 10:eaaf4736. Moogk D, et al. 2016. J Immunol. 197:644. Klammt C, et al. 2015. Nat Immunol. 16:961. Cancer Genome Atlas Network. 2015. Cell. 161:1681. Casas J, et al. 2014. Nat Commun. 5:5624. McNeill L, et al. 2007. Immunnity. 27:425. Lefebvre DC, et al. 2003. Biochim Biophys Acta. 1650:40. Kabouridis PS. et al. 2003. Biochem J. 371:907. Abraham N and Veillette 1990. Mol. Cell. Biol. 10:5197.
Gene ID: 3932
UniProt: View information about Lck on UniProt.org
Clone: LCK-01; A18002D;
Regulatory Status: RUO
Other Names: LSK; 56lck; LCK Proto-Oncogene, Src Family Tyrosine Kinase; p56LCK; Pp58Lck T Cell-Specific Protein Tyrosine Kinase