Biolegend, 699956, PhosphoPair PLK-1 (Thr210) Antibody Set, 1kit

PLK-1 (polo-like kinase 1) is a member of the Polo-like kinase family of serine/threonine protein kinases. While PLK-1 phosphorylates a broad range of targets, it has been shown to be a critical regulator of cell cycle pathways, ranging from mitotic entry to initiation of cytokinesis. Elevated PLK1 expression is described in many cancers and is associated with poor clinical outcomes. Increased PLK-1 activity is associated with resistance to doxorubicin and gemcitabine in certain cancers. Activation of PLK-1 requires phosphorylation of Thr210 within the T-loop of the catalytic domain by Aurora kinases.
1kit
Verified Reactivity: Human
Immunogen: Please refer to individual product datasheets for immunogen information
Formulation: Phosphate-buffered solutions, pH 7.2, containing 0.09% sodium azide
Preparation: The antibodies were purified by affinity chromatography.
Storage & Handling: The antibody solutions should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of antibodies in this set is quality control tested by Western blotting. For Western blotting, the suggested uses of these reagents are as follows: Clone 3F8: 0.2 - 1.0 µg/mL Clone 2A3: 0.5 - 1.0 µg/mL It is recommended that the reagent be titrated for optimal performance for each application. For recommended usage information for additional applications, please refer to the datasheet of the individual products.
Product Citations: Chilamakuri R, et al. 2022. Pharmaceuticals (Basel). 15:. PubMed
Structure: PLK1 is a 603 amino acid protein with a predicted molecular weight of ~68 kD.
Distribution: Ubiquitously expressed; highly expressed in placenta and colon/Centrosomes and kinetochore
Function: Mitotic signaling
Antigen References: 1. Hamanaka R, et al. 1994. Cell Growth Differ. 5:249. 2. Lake RJ, et al. 1993. Mol. Cell. Biol. 13:7793. 3. Holtrich U, et al. 1994. P. Natl. Acad. Sci. USA 91:1736.
Gene ID: 5347
UniProt: View information about PLK-1 on UniProt.org
Clone: 3F8; 2A3;
Regulatory Status: RUO
Other Names: Polo Like Kinase 1,STPK13, Cell Cycle Regulated Protein Kinase