Biolegend, 717455, Recombinant Human Frizzled-4-Fc Chimera (carrier-free), 50μg

CD344 is a member of the Frizzled family, also known as Frizzled-4 (FZD-4). Ten Frizzled family members have been identified in humans. According to the identity of amino acid sequences, frizzled proteins can be divided into four subgroups: subfamily 1 (FZD-1, FZD-2, and FZD-7), subfamily 2 (FZD-5 and FZD-8), subfamily 3 (FZD-9 and FZD-10), subfamily 4 (FZD-4) and subfamily 5 (FZD-3 and FZD-6). The human FZD-4 gene is located on chromosome 11q14–q21. FZD-4 is a 48–53 kDa seven-transmembrane G protein–coupled receptor (GPCR) that serves as a receptor for Wnt proteins and Norrin. In the presence of Wnt ligand, Wnt binds to extracellular cysteine rich domain (CRD) of Frizzled. A receptor complex forms between Frizzled and low-density lipoprotein receptor-related protein 5/6 (LRP5/6). Norrin, an atypical Wnt ligand, binds to FZD-4 and LRP5/6, activating the canonical Wnt/β-catenin pathway (canonical Wnt signaling). FZD-4 is a neuronal stem cell marker and is also expressed in fetal neuronal progenitor cells and intestinal neuronal cells. High levels of FZD-4 expression are found in the kidney, lung, brain, and liver. FZD-4 has been reported to regulate retinal and inner ear angiogenesis and plays a role in embryonic development, tissue and cell polarity, and cell proliferation.
50μg
Source: Recombinant human Frizzled-4, amino acid Phe37-Glu180 (Accession No. Q9ULV1), with a proline at N-terminus and a C-terminal Xa-mutant hFc (C103S), was expressed in CHO cells.
Molecular Mass: The 382 amino acid recombinant protein has a predicted molecular mass of approximately 43.0 kD. The DTT-reduced and non-reduced proteins migrate at approximately 50 - 62 kD and 80 - 115 kD by SDS-PAGE respectively. The N-terminal amino acid is Pro.
Purity: > 95%, as determined by Coomassie stained SDS-PAGE.
Formulation: 0.22 µm filtered protein solution is in PBS, pH 7.2
Endotoxin Level: Less than 0.1 EU per µg protein as determined by the LAL method.
Concentration: 50 µg size is bottled at 0.2 mg/mL
Storage & Handling: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity: ED50= 0.25 - 2.5 µg/mL as measured by its ability to inhibit Topflash reporter activity in HEK293T human embryonic kidney cells induced by recombinant human Wnt3a.
Application: Bioassay
Application Notes: BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected].
Structure: Dimer
Distribution: Kidney, lung, brain, liver, neuronal stem cells, fetal neuronal progenitor cells and neuronal intestinal cells
Function: Receptor for Wnt proteins that function in the canonical Wnt/beta-catenin signaling pathway, embryonic development, tissue/cell polarization and proliferation
Ligand/Receptor: Norrin, Wnt proteins
Bioactivity: Measured by its ability to inhibit recombinant Wnt3a activity in Topflash assay
Biology Area: Angiogenesis, Cell Biology, Immunology
Molecular Family: CD Molecules
Antigen References: Qian Y, et al. 2024. Nat Commun. 15:7644. Kushwaha P, et al. 2020. J Cell Physiol. 235:6673. Caceres L, et al. 2019. Dev Dyn. 248:1243. Neckel PH, et al. 2019. Cells. 8:792. DeBruine ZJ, et al. 2017. Genes Dev. 31:916. Rickman M, et al. 2023. J Cell Sci. 136: jcs260449. Descamps B, et al. 2012. Circ Res. 110:47. Shen G, et al. 2012. Cell Res. 25:1078.
Gene ID: 8322
UniProt: View information about Frizzled-4 on UniProt.org
Regulatory Status: RUO
Other Names: Frizzled4, Frizzled-4, FZ-4, FZD4, FZD4S, FzE4, hFZ4, CD344, CD344 antigen,Frizzled (Drosophila) homolog 4, EVR1, GPCR, WNT receptor frizzled-4
Q: Why choose BioLegend recombinant proteins?
A: • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
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Q: How does the activity of your recombinant proteins compare to competitors?
A: We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
Q: What is the specific activity or ED50 of my recombinant protein?
A: The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
Q: Have your recombinants been tested for stability?
A: Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
Q: Does specific activity of a recombinant protein vary between lots?
A: Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
Q: How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
A: Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)