Biolegend, 740446, LEGENDplex™ Mouse Inflammation Panel (13-plex) with V-bottom Plate, 100tests

Cytokines are small soluble glycoproteins that act as extracellular signaling molecuels and meidate communications between cells. They regulate immunity, inflammation and hematopoiesis as well as cell growth, migration, development and differentiation. Cytokines act in large tightly regulated networks where both the production and action of one cytokine is affected by the behavior of other cytokines. The accurate measurement of the expression profile of cytokines in a sample is critical to complete and in-depth understanding of disease progression and immune responses. The LEGENDplex™ Mouse Inflammation Panel is a multiplex assay by using fluorescence–encoded beads suitable for the analysis on various flow cytometers. This panel allows simultaneous quantification of 13 mouse cytokines, including IL-1α, IL-1β, IL-6, IL-10, IL-12p70, IL-17A, IL-23, IL-27, MCP-1, IFN-β, IFN-γ, TNF-α, and GM-CSF. Most of cytokines in this panel are produced by innate immune cells, linking the innate and adaptive immunity and/or bystander cells. This assay panel provides higher detection sensitivities and broader dynamic ranges than traditional ELISA methods. The panel has been validated by detecting expected changes in biological samples. A LEGENDplex™ Mix and Match system is available to customize assays for specificities within this panel.
100tests
Verified Reactivity: Mouse
Application: Multiplex Learn more about LEGENDplex™ at biolegend.com/legendplex Download the LEGENDplex™ software here.
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Biology Area: Cell Biology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Gene ID: 35533439344034587124356935763586359235933605360635935156190865
Regulatory Status: RUO
Other Names: IL-23, IL-1α, IFN-γ, TNF-α, CCL2 (MCP-1), IL-12p70, IL-1β, IL-10, IL-6, IL-27, IL-17A, IFN-β, GM-CSF
Q: If I don't have a vacuum, how do I remove the liquid from my plate?
A: If you do not have a vacuum, the assay should be run in a V-bottom plate. After centrifugation using a swinging-bucket rotor with a plate adaptor, you can remove the liquid by flicking the plate quickly, dumping the contents into a sink, and patting it dry carefully on a stack of clean paper towels without losing the beads. Alternatively, you can remove the liquid by using a pipette.
Q: Should I perform the assay with the filter plates or with V-bottom plates?
A: Filter plates or V-bottom plates have been included in some kits for your convenience. A vacuum filtration unit is required to work with the filter plates. However, if you don’t have access to a vacuum manifold or if you prefer, then you can use the V-bottom plates and follow the recommended assay protocols for the type of plates you choose. All plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.
Q: After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?
A: The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.
Q: What is the shelf life of LEGENDplex™ kits?
A: LEGENDplex™ kits are guaranteed for 6 months from the date of receipt, but may have a shelf life of up to 2 years from the date of manufacture.
Q: Is special software required for data analysis?
A: Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). Other software may be available to analyze FCS files. Data generated using LEGENDplex™ kits can be analyzed using the freely available LEGENDplex™ data analysis software. Please check our website for the most updated versions of the software.