Biolegend, 741158, LEGENDplex™ HU Proinflam. Chemokine Panel 2 (12-plex) w/ VbP, 100tests

Chemotactic cytokines or chemokines play pivotal roles in various processes such as immune surveillance, organ development, angiogenesis, and immune responses.  Expression profiling of chemokines, especially those involved in inflammation and immune disorders, is important in achieving a deeper understanding of disease states. The Human Proinflammatory Chemokine Panel 2 is a multiplex bead-based assay panel, using fluorescence–encoded beads suitable for use on various flow cytometers. This panel allows simultaneous quantification of 12 human chemokines, including CCL1 (I-309), CCL13 (MCP-4), CCL22 (MDC), CCL7 (MCP-3), CCL24 (Eotaxin-2), CCL19 (ELC), CXCL13 (BLC), CCL8 (MCP-2), CX3CL1 (Fractalkine), CXCL12 (SDF-1),  CXCL2(GRO-β) and CCL18 (PARC). This assay panel provides higher detection sensitivities and broader dynamic ranges than traditional ELISA methods. The panel has been validated by detecting expected changes in biological samples. The panel has been validated for use on cell culture supernatant and serum samples. Use with caution for plasma samples. The LEGENDplex™ Human Proinflammatory Chemokine Panel 2 (12-plex) is designed to allow flexible customization within the panel. It can also be divided into subpanels. The table below shows the panel configuration and sample dilution requirement. Cat # Plex Size Targets Sample Type Dilution Factor 741157, 741158 12-plex CCL1, CCL13, CCL22, CCL7, CCL24, CCL19, CX CL13, CCL8, CX3CL1, CXCL12, CXCL2, CCL18 Tissue Culture Varies 741182, 741183 11-plex CCL1, CCL13, CCL22, CCL7, CCL24, CCL19, CX CL13, CCL8, CX3CL1, CXCL12, CXCL2 Tissue Culture Varies Serum/Plasma 2X 741184, 741185 1-plex CCL18 Tissue Culture Varies Serum/Plasma 50X Please visit www.biolegend.com/legendplex for more information on how to mix and match within the panel.
100tests
Verified Reactivity: Human
Application: Multiplex Learn more about LEGENDplex™ at biolegend.com/legendplex Download the LEGENDplex™ software here.
Gene ID: 6346635763676354636963631056363556376638729206362
Regulatory Status: RUO
Other Names: CCL1 (I-309), CCL13 (MCP-4), CCL22 (MDC), CCL7 (MCP-3), CCL24 (Eotaxin-2), CCL19 (ELC), CXCL13 (BLC), CCL8 (MCP-2), CX3CL1 (Fractalkine) CXCL12 (SDF-1), CXCL2 (GRO-β), CCL18 (PARC)
Q: If I don't have a vacuum, how do I remove the liquid from my plate?
A: If you do not have a vacuum, the assay should be run in a V-bottom plate. After centrifugation using a swinging-bucket rotor with a plate adaptor, you can remove the liquid by flicking the plate quickly, dumping the contents into a sink, and patting it dry carefully on a stack of clean paper towels without losing the beads. Alternatively, you can remove the liquid by using a pipette.
Q: Should I perform the assay with the filter plates or with V-bottom plates?
A: Filter plates or V-bottom plates have been included in some kits for your convenience. A vacuum filtration unit is required to work with the filter plates. However, if you don’t have access to a vacuum manifold or if you prefer, then you can use the V-bottom plates and follow the recommended assay protocols for the type of plates you choose. All plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.
Q: After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?
A: The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.
Q: What is the shelf life of LEGENDplex™ kits?
A: LEGENDplex™ kits are guaranteed for 6 months from the date of receipt, but may have a shelf life of up to 2 years from the date of manufacture.
Q: Is special software required for data analysis?
A: Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). Other software may be available to analyze FCS files. Data generated using LEGENDplex™ kits can be analyzed using the freely available LEGENDplex™ data analysis software. Please check our website for the most updated versions of the software.