Biolegend, 741294, LEGENDplex™ MU Proinflam. Chemokine Panel 1 (13-plex) w/FP V02, 100tests

Chemokines play pivotal roles in various processes such as immune surveillance, organ development, angiogenesis, and immune responses. Expression profiling of chemokines, especially those involved in inflammation and immune disorders, is important in achieving a deeper understanding of disease states. The LEGENDplex™ Mouse Proinflammatory Chemokine Panel 1 V02 is a multiplex bead-based assay intended for use on various flow cytometers. This panel allows simultaneous quantification of 13 mouse chemokines, including CXCL1 (KC), CCL2 (MCP-1), CCL5 (RANTES), CXCL10 (IP-10), CCL11 (Eotaxin), CCL17 (TARC), CCL3 (MIP-1α), CCL4 (MIP-1β), CXCL9 (MIG), CCL20 (MIP-3α), CXCL5 (LIX), CXCL13 (BLC) and CCL22 (MDC). This panel provides higher sensitivities and broader dynamic ranges than the traditional ELISA method. The panel has been validated for use on serum/plasma and cell culture supernatant samples. A LEGENDplex™ Mix and Match system is available to customize assays for specificities within this chemokine panel.
100tests
Verified Reactivity: Mouse
Application: Multiplex - Panel Learn more about LEGENDplex™ at biolegend.com/legendplex Download the LEGENDplex™ software here.
Molecular Family: Cytokines/Chemokines
Gene ID: 20304202972029220311202991482520302173291594520303202952029655985
Regulatory Status: RUO
Other Names: CXCL1 (KC), CCL5 (RANTES), CCL20 (MIP-3a), CCL11 (Eotaxin), CCL17 (TARC), CCL2 (MCP-1), CXCL9 (MIG), CXCL10 (IP-10), CCL3 (MIP-1a), CCL4 (MIP-1b), CXCL13 (BLC), CXCL5 (LIX), CCL22 (MDC)
Q: If I don't have a vacuum, how do I remove the liquid from my plate?
A: If you do not have a vacuum, the assay should be run in a V-bottom plate. After centrifugation using a swinging-bucket rotor with a plate adaptor, you can remove the liquid by flicking the plate quickly, dumping the contents into a sink, and patting it dry carefully on a stack of clean paper towels without losing the beads. Alternatively, you can remove the liquid by using a pipette.
Q: Should I perform the assay with the filter plates or with V-bottom plates?
A: Filter plates or V-bottom plates have been included in some kits for your convenience. A vacuum filtration unit is required to work with the filter plates. However, if you don’t have access to a vacuum manifold or if you prefer, then you can use the V-bottom plates and follow the recommended assay protocols for the type of plates you choose. All plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.
Q: After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?
A: The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.
Q: What is the shelf life of LEGENDplex™ kits?
A: LEGENDplex™ kits are guaranteed for 6 months from the date of receipt, but may have a shelf life of up to 2 years from the date of manufacture.
Q: Is special software required for data analysis?
A: Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). Other software may be available to analyze FCS files. Data generated using LEGENDplex™ kits can be analyzed using the freely available LEGENDplex™ data analysis software. Please check our website for the most updated versions of the software.