Biolegend, 741562, LEGENDplex™ Human Neuroinflammation Panel 2 (10-plex) w/ VbP, 100tests

Neuroinflammation is the condition in which cells in the central nervous system - including astrocytes, microglia, and endothelial cells - respond to injury, infection, or pro-inflammatory signals by secreting cytokines and chemokines. These signaling molecules further amplify the inflammatory response, potentially leading to neuronal death, followed by neurogenesis and tissue repair. In cases of acute neuroinflammation, such as stroke, traumatic brain injury, meningitis, or encephalomyelitis, an excessive inflammation response can have detrimental effects such as neuronal damage and neurodegeneration. Additionally, incomplete resolution of inflammation may result in chronic neuroinflammation. Chronic neuroinflammation, whether arising from a prior acute episode or occurring without a clear cause, is implicated in the pathogenesis of neurodegenerative di  Inflammation of the central nervous system can weaken the blood-brain barrier (BBB), allowing peripheral immune cells to infiltrate the brain or enabling inflammatory signals to spread systemically. As a result, elevated concentrations of inflammatory cytokines can be detected not only in the cerebrospinal fluid (CSF) but also in the serum and plasma. The LEGENDplex™ Human Neuroinflammation Panel 2 is designed to quantify beneficial and detrimental aspects of neuroinflammation by measuring cytokines that often elevated in serum, plasma, and/or CSF of patients with neuroinflammation conditions. The LEGENDplex™ Human Neuroinflammation Panel 2 (10-plex) is a bead-based multiplex assay intended for use on various flow cytometers. This panel allows simultaneous quantification of 10 neuroinflammation markers: CD14, CD163, CRP (PTX1), D-dimer, ICAM-1 (CD54), MMP-2, NSE (ENO2), VCAM-1 (CD106), vWF, YKL-40 (CHI3L1). This panel provides broader dynamic range than traditional ELISA methods and allows simultaneous detection of several cytokines in a sample. The panel has been validated for use on serum, plasma, CSF, and cell culture supernatant samples. The LEGENDplex™ Human Neuroinflammation Panel 2 (10-plex) is designed to allow flexible customization within the panel. Please visit https://www.biolegend.com/en-us/immunoassays/legendplex for more information on how to mix and match within the panel. This assay is for research use only.
100tests
Verified Reactivity: Human
Application: Multiplex - Panel Learn more about LEGENDplex™ at biolegend.com/legendplex Download the LEGENDplex™ software here.
Cell Type: Astrocytes, Macrophages, Neurons
Biology Area: Neurodegeneration, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Signal Transduction
Molecular Family: Cytokines/Chemokines
Gene ID: NA
Regulatory Status: RUO
Other Names: sCD14, CD163, CRP (PTX1), D-dimer, ICAM-1 (CD54), MMP-2, NSE (ENO2) , VCAM-1 (CD106), vWF, YKL-40 (CHI3L1)
Q: If I don't have a vacuum, how do I remove the liquid from my plate?
A: If you do not have a vacuum, the assay should be run in a V-bottom plate. After centrifugation using a swinging-bucket rotor with a plate adaptor, you can remove the liquid by flicking the plate quickly, dumping the contents into a sink, and patting it dry carefully on a stack of clean paper towels without losing the beads. Alternatively, you can remove the liquid by using a pipette.
Q: Should I perform the assay with the filter plates or with V-bottom plates?
A: Filter plates or V-bottom plates have been included in some kits for your convenience. A vacuum filtration unit is required to work with the filter plates. However, if you don’t have access to a vacuum manifold or if you prefer, then you can use the V-bottom plates and follow the recommended assay protocols for the type of plates you choose. All plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.
Q: After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?
A: The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.
Q: What is the shelf life of LEGENDplex™ kits?
A: LEGENDplex™ kits are guaranteed for 6 months from the date of receipt, but may have a shelf life of up to 2 years from the date of manufacture.
Q: Is special software required for data analysis?
A: Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). Other software may be available to analyze FCS files. Data generated using LEGENDplex™ kits can be analyzed using the freely available LEGENDplex™ data analysis software. Please check our website for the most updated versions of the software.