Biolegend, 773301, Prime-Step™ Prestained Broad Range Protein Ladder, 50microl

The Prime-Step™ Prestained Broad Range Protein Ladder is a three-color protein standard with 10 pre-stained proteins covering a wide range of molecular weights from 6.5 to 270 kDa. It has 7 blue-stained bands and 3 reference bands (2 orange bands at 30 kDa and 270 kDa and 1 green band at 52 kDa) when separated on Tris-glycine gel. The Prime-Step™ Prestained Broad Range Protein Ladder is designed for monitoring protein separation during SDS-PAGE electrophoresis, confirming Western blot transfer quality on membranes (PVDF or nitrocellulose membrane) and for approximating the size of target proteins on the gel or blot. Key Product Features
50microl
Formulation: Sample loading buffer (62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3, 33% glycerol).
Preparation: Chromophore conjugated recombinant protein mixture supplied in sample loading buffer.
Storage & Handling: Upon receipt, store at -20°C.
Application: WB - Quality tested
Recommended Usage: • 3-5 μl per loading for clear visualization during electrophoresis on 15-well mini-gel or 20-well midi-gel, respectively. • 3-5 μl per well for general Western transferring. The ladder is supplied in the sample loading buffer and is ready to use.
Application Notes: The apparent molecular weight (kDa) of each protein has been determined by calibration against unstained protein standards.
Biology Area: Cell Biology
Gene ID: NA
Regulatory Status: RUO
Other Names: protein ladder, protein marker, pre-stained protein ladder, pre-stained protein marker, protein molecular weight marker