Biolegend, 775314, Cell-Vive™ GMP Recombinant Human FN-1 (carrier-free), 25μg

Fibronectin (FN) is an adhesive glycoprotein found as insoluble components of the extracellular matrix (ECM) or as soluble dimeric forms in plasma. Fibronectin binds chondroitin sulfate, collagen, fibrin, heparin, and many integrin receptors through which it mediates multiple cellular signaling pathways. Conversion of soluble fibronectin to fibronectin fibrils is initiated by binding to cell surface integrins. Fibronectin is involved in cell adhesion, cell motility, wound healing and maintenance of cell morphology. It also participates in osteoblast compaction and is essential for osteoblast mineralization. Fibronectin is thought to play a role in platelet thrombus formation and other diseases associated with thrombosis including atherosclerosis and cardiac repair following a myocardial infarct. Fibronectin plays an important role in coordinating interaction between the ECM and cancer cells in tumors. Its upregulation enhances cancer cell survival, proliferation, and invasion. Fibronectin monomers consist of three types of homologous repeats, type I, II and III domains. A fragment from the first type III repeat, known as Anastellin, binds to fibronectin and induces fibril formation named superfibronectin. Superfibronectin exhibits enhanced adhesive properties and inhibits tumor growth, angiogenesis, and metastasis. BioLegend Cell-Vive™ GMP Recombinant proteins are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12 in a dedicated GMP facility compliant with ISO 13485:2016. Specifications and processes include:
25μg
Source: Human NF-1, amino acid (Gln32-Pro1991) (Accession: # BC117176) with a C-terminal 8His-GGQ tag was expressed in 293E cells.
Molecular Mass: The 1973 amino acid recombinant protein has a predicted molecular mass of approximately 217 kD. The DTT-reduced and non-reduced protein migrates at approximately 180 – 220 kD by SDS-PAGE. The predicted N-terminal amino acid is Gln.
N-terminalSequence Analysis: Gln-Pro-Ala-Pro-Val-Val-Asp-Leu-Arg-Pro
Purity: ≥ 95%, as determined by Coomassie stained SDS-PAGE
Formulation: 0.1 µm filtered protein solution is in PBS, pH 7.2.
Endotoxin Level: Less than or equal to 0.1 EU per μg protein as determined by the LAL method.
Residual Host Cell Protein Content: ≤ 0.500 ng/μg by ELISA
Concentration: 0.5 mg/mL
Storage & Handling: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week or stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity: Immobilized recombinant human FN-1 supports the adhesion of mouse L929 cells in a dose dependent manner. The ED50 for this effect is 15 – 60 ng/mL.
Application: Bioassay Cell Culture
Application Notes: BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected].
Disclaimer: BioLegend Cell-Vive™ GMP Recombinant proteins are for research use only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.
Structure: Monomer
Distribution: Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial, and other cell types, is deposited as fibrils in the extracellular matrix.
Function: Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin
Interaction: Cell surface, collagen, fibrin, heparin, DNA, and actin
Ligand/Receptor: Integrins α5β1, α4β1, α4β7, α9β1, αvβ3; non-integrin receptors TLR-4, CD44, Syndecan-4, collagen, fibrin, heparin, DNA, and actin
Bioactivity: FN-1 supports the adhesion of mouse L929 cells.
Biology Area: Angiogenesis, Cancer Biomarkers, Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience Cell Markers
Antigen References: Schwarz-Linek U, et al. 2004. Mol Microbiol. 52:631. Nagai Y, et al. 2006. Immunity. 24:801. White ES, et al. 2008. J. Pathol. 216:1. Adams JC, et al. 2015. Cell Adh Migr. 9:22. Kraft S, et al. 2016. J. Mol. Med. 94:567. Speziale P, et al. 2019. Cells. 8:1516. Wirth F, et al. 2020. Cells. 9:2717.
Gene ID: 2335
UniProt: View information about FN-1 on UniProt.org
Other Names: Fibronectin, FN, FN1, Cold-insoluble globulin, CIG, Large external transformation-sensitive protein, LETS