Biolegend, 803302, Purified anti-Apo E Antibody (Previously Covance catalog# SIG-39740), 500microl

Apo-E is a class of apolipoprotein primarily synthesized in the liver, but is also found in other tissues such as brain, kidneys, and spleen. Apo-E is a protein component of serum chylomicron and lipoprotein particles, playing essential roles in cholesterol transportation, homeostasis, and metabolism. The expression of Apo-E is induced by cholesterol-rich diets and hyperlipoproteinemia. In the central nervous system, Apo-E is produced by astrocytes and microglia. The Apo-E-containing lipoprotein particles play important functions in brain injury repair, neurogenesis, and synaptic plasticity. Apo-E has been found to be involved in the regulation of amyloid-β aggregation and clearance. Genetic polymorphisms of Apo-E are associated with neuron degenerative disorders including Alzheimer's disease.
500microl
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This antibody was raised against a peptide sequence corresponding to aa 141-160 of human Apo-E.
Formulation: Phosphate-buffered solution (no preservatives or carrier proteins).
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1 mg/ml
Storage & Handling: This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application: WB - Quality tested IHC-P, ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5 - 10 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.0 - 10 is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Additional Product Notes: For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with any of the following: -Fixation buffer (Cat. No. 420801) with 0.5 % Triton X-100 -Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol -100% ice-cold methanol only Fixation and permeabilization with 100% ice-cold methanol results in dimmer signal compared to the other methods. This product is not recommended for use in intracellular flow cytometry (ICFC)
Application References(PubMed link indicates BioLegend citation): Munoz DG and Feldman H. 2000. CMAJ. 162(1):65-72. Hagberg JM, et al. 2000. Physiol Genomics. 4(2):101-108. Saunders AM. 2000. J Neuropathol Exp Neurol. 59(9):751-8. Golabek AA, et al. 2000. Biophys J. 79(2):1008-15. Zunarelli E, et al. 2000. Breast Cancer Res Treat. 63(3):193-8. Zunarelli E, et al. 2000. Clin Neuropathol. 19(1):1-6. Tomiyama T, et al. 1999. Cell Mol Life Sci. 6(3-4):268-79. Golabek AA., et al. 1996. J Biol Chem. 271(18):10602-6.
Product Citations: Fote GM, et al. 2022. J Cell Sci. 135:. PubMed McQuade A, et al. 2020. Nat Commun. 4.1875. PubMed
RRID: AB_2564662 (BioLegend Cat. No. 803301) AB_2564661 (BioLegend Cat. No. 803303)
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Gene ID: 348
UniProt: View information about Apo E on UniProt.org
Clone: D6E10
Regulatory Status: RUO
Other Names: Apolipoprotein E, apolipoprotein E3, AD2, LDLCQ5, LPG, ApoE
Previously: Signet Catalog# 9740-02Signet Catalog# 9740-05Signet Catalog# 9740-10Covance Catalog# SIG-39740
Isotype: Mouse IgG1, κ